Johansson M E, Wadell G
J Immunol Methods. 1978;19(2-3):259-67. doi: 10.1016/0022-1759(78)90185-0.
The conditions for preparation of adenovirus antisera with group, subgroup and type-specific reactivity to be used in the immunofluorescence technique have been evaluated. Group-specific antibodies were prepared by passing an antiserum against virions or hexons of one serotype through an immunosorbent column containing soluble viral components of an adenovirus type belonging to a different subgroup. The group-specific antibodies were recovered from the solid phase by elution at pH 2.8. Reagents specific for subgroup I and II were obtained by passing anti-dodecon sera through an immunosorbent column containing soluble viral components from heterologous subgroups. In this case antibodies not attaching to the absorbent were recovered. Sera against fibers of subgroup III members could be used as subgroupspecific sera without absorption. Type-specific antibodies were prepared by removal of antibodies of other specificities by passing anti-virion or anti-hexon sera through immunosorbent columns containing soluble viral components of an adenovirus type belonging to the same subgroup. Reagents specific for seven adenovirus types representing Rosen's three subgroups were prepared according to the outlined procedures.
已评估了用于免疫荧光技术的具有组、亚组和型特异性反应性的腺病毒抗血清的制备条件。通过将针对一种血清型病毒粒子或六邻体的抗血清通过含有属于不同亚组的腺病毒型可溶性病毒成分的免疫吸附柱来制备组特异性抗体。通过在pH 2.8下洗脱从固相中回收组特异性抗体。通过将抗十二聚体血清通过含有来自异源亚组的可溶性病毒成分的免疫吸附柱来获得针对I和II亚组的特异性试剂。在这种情况下，回收未附着于吸附剂的抗体。针对III亚组成员纤维的血清无需吸收即可用作亚组特异性血清。通过将抗病毒粒子或抗六邻体血清通过含有属于同一亚组的腺病毒型可溶性病毒成分的免疫吸附柱来去除其他特异性抗体，从而制备型特异性抗体。根据所述程序制备了代表罗森三个亚组的七种腺病毒型的特异性试剂。
