Block S M, Svoboda K
Department of Molecular Biology, Princeton University, New Jersey 08544, USA.
Biophys J. 1995 Apr;68(4 Suppl):230S-239S; discussion 239S-241S.
The development of in vitro motility assays for motor proteins has been accompanied by a parallel development of advanced optical instrumentation capable of recording motion at the molecular level. Devices now exist that can record displacements to better than 0.1 nm at bandwidths in excess of 10 kHz, and that can place controlled forces up to many pN on single motors. Ultra-high resolution data from experiments are now pouring in. The analysis and subsequent interpretation of experimental records, which are inevitably contaminated with high levels of thermal noise, remain an ongoing challenge. This essay examines selected issues relating to this challenge and discusses some alternative approaches.
用于运动蛋白的体外运动分析方法的发展，一直伴随着能够在分子水平记录运动的先进光学仪器的同步发展。现在已有能在超过10kHz的带宽下记录精度优于0.1nm的位移，且能对单个马达施加高达数皮牛控制力的设备。来自实验的超高分辨率数据正不断涌现。对不可避免地被高水平热噪声污染的实验记录进行分析和后续解读，仍然是一项持续的挑战。本文探讨了与这一挑战相关的一些问题，并讨论了一些替代方法。
