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通过限制性片段分析对酿酒酵母2μm DNA进行表征并整合到大肠杆菌质粒中。

Characterization of 2-mum DNA of Saccharomyces cerevisiae by restriction fragment analysis and integration in an Escherichia coli plasmid.

作者信息

Hollenberg C P, Degelmann A, Kustermann-Kuhn B, Royer H D

出版信息

Proc Natl Acad Sci U S A. 1976 Jun;73(6):2072-6. doi: 10.1073/pnas.73.6.2072.

DOI:10.1073/pnas.73.6.2072
PMID:778854
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC430451/
Abstract

Electrophoretic analysis of EcoRI and HindIII restriction fragments of 2-mum supercoiled DNA of Saccharomyces cerevisiae indicated that this class of DNA is heterogeneous and probably consists of two types of molecules. Integration of the 2-mum yeast DNA in E. coli plasmid pCR1 directly showed that existence of two types of molecules as each of these could be individually inserted into separate bacterial plasmids. The difference between the two types of 2-mum circles is due to an inversion of about 1.6 X 10(6) daltons. The inversion is flanked by a reversed duplicated sequence of 0.45 X 10(6) daltons. Possible implications of this structure are dicussed.

摘要

对酿酒酵母2μm超螺旋DNA的EcoRI和HindIII限制性片段进行的电泳分析表明，这类DNA是异质的，可能由两种类型的分子组成。将2μm酵母DNA整合到大肠杆菌质粒pCR1中直接表明存在两种类型的分子，因为每一种都可以单独插入到不同的细菌质粒中。两种类型的2μm环之间的差异是由于约1.6×10⁶道尔顿的倒位。该倒位两侧是0.45×10⁶道尔顿的反向重复序列。讨论了这种结构可能的影响。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8de0/430451/4b59edd3e663/pnas00036-0309-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8de0/430451/2ff97f0ded1c/pnas00036-0307-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8de0/430451/30ff1b96a114/pnas00036-0308-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8de0/430451/64da78ed2fac/pnas00036-0308-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8de0/430451/4b59edd3e663/pnas00036-0309-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8de0/430451/2ff97f0ded1c/pnas00036-0307-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8de0/430451/30ff1b96a114/pnas00036-0308-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8de0/430451/64da78ed2fac/pnas00036-0308-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8de0/430451/4b59edd3e663/pnas00036-0309-a.jpg

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Characterization of 2-mum DNA of Saccharomyces cerevisiae by restriction fragment analysis and integration in an Escherichia coli plasmid.通过限制性片段分析对酿酒酵母2μm DNA进行表征并整合到大肠杆菌质粒中。
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引用本文的文献

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