Sutton L D, Biedenbach D J, Yen A, Jones R N
Department of Pathology, University of Iowa College of Medicine, Iowa City 52242, USA.
Diagn Microbiol Infect Dis. 1995 Jan;21(1):1-8. doi: 10.1016/0732-8893(94)00075-8.
A novel, chromogenic cephalosporin reagent (S1) for beta-lactamase testing was produced that shares physicochemical characteristics with nitrocefin (formerly 87/312). S1 and nitrocefin in a disk-testing format for beta-lactamase performed at 100% agreement for detecting enzyme-producing isolates of Bacteroides fragilis group, Haemophilus influenzae, Moraxella catarrhalis, Neisseria gonorrhoeae, Staphylococcus aureus, and selected Enterobacteriaceae. The time required to achieve an initial color change or a strong positive reaction was comparable for both chromogenic reagents for all organisms except the Gram-positive species. S1 reaction times were approximately 50% faster than nitrocefin for beta-lactamase-positive enterococci and S. aureus. These results from the developmental studies and a commercially prepared disk lot indicate that S1 is a promising beta-lactamase disk test reagent with the ability to detect all significant enzyme-producing species strains, some significantly earlier than the nitrocefin disk method.
研制出了一种用于β-内酰胺酶检测的新型显色头孢菌素试剂（S1），它具有与硝噻吩（原87/312）相同的物理化学特性。在检测β-内酰胺酶的纸片试验中，S1和硝噻吩在检测脆弱拟杆菌属、流感嗜血杆菌、卡他莫拉菌、淋病奈瑟菌、金黄色葡萄球菌及部分肠杆菌科产酶菌株时，符合率达100%。除革兰氏阳性菌外，对于所有微生物，两种显色试剂达到初始颜色变化或强阳性反应所需的时间相当。对于β-内酰胺酶阳性的肠球菌和金黄色葡萄球菌，S1的反应时间比硝噻吩快约50%。这些研发研究结果及一批市售纸片表明，S1是一种很有前景的β-内酰胺酶纸片检测试剂，能够检测所有重要的产酶菌株，而且在某些情况下比硝噻吩纸片法能更早地检测到。
