Barton R C, van Belkum A, Scherer S
Human Genome Center, Lawrence Berkeley Laboratory, Berkeley, California 94720, USA.
J Clin Microbiol. 1995 Apr;33(4):794-6. doi: 10.1128/jcm.33.4.794-796.1995.
Serial isolates of Candida albicans were obtained from 20 patients with leukemia over periods of up to 8 months. The fingerprinting of these isolates by interrepeat PCR and random amplified polymorphic DNA PCR has been described previously (A. van Belkum, W. Melchers, B. E. de Pauw, S. Scherer, W. Quint, and J. F. Meis, J. Infect. Dis. 169:1062-1070, 1994). Contour-clamped homogeneous electric field gel electrophoresis was used to examine the chromosomes of these isolates. When changes in the karyotype were seen in a series of isolates, additional interrepeat PCR and Southern blotting with a repeat DNA sequence from the 27A family were performed. These two genotyping tools were used to determine if karyotypic changes seen in a series of isolates were due to chromosome rearrangements in a single strain or due to colonization with more than one strain. It was determined that changes in karyotype in a series of strains indicated infection by a new strain.
从20例白血病患者中获取白色念珠菌的系列分离株，时间长达8个月。此前已描述过通过重复序列间PCR和随机扩增多态性DNA PCR对这些分离株进行指纹图谱分析（A. van Belkum、W. Melchers、B. E. de Pauw、S. Scherer、W. Quint和J. F. Meis，《传染病杂志》169:1062 - 1070，1994年）。采用轮廓夹恒定电场凝胶电泳来检测这些分离株的染色体。当在一系列分离株中观察到核型变化时，会进一步进行重复序列间PCR以及用来自27A家族的重复DNA序列进行Southern印迹分析。这两种基因分型工具用于确定在一系列分离株中观察到的核型变化是由于单个菌株中的染色体重排，还是由于感染了不止一个菌株。结果确定，一系列菌株中的核型变化表明感染了新菌株。
