一种新型革兰氏阴性杆菌鉴定系统——Crystal肠道菌/非发酵菌鉴定系统的评估。
Evaluation of a new identification system, Crystal Enteric/Non-Fermenter, for gram-negative bacilli.
作者信息
Wauters G, Boel A, Voorn G P, Verhaegen J, Meunier F, Janssens M, Verbist L
机构信息
Microbiology Unit, University of Louvain, Brussels, Belgium.
出版信息
J Clin Microbiol. 1995 Apr;33(4):845-9. doi: 10.1128/jcm.33.4.845-849.1995.
A total of 505 fermentative and 201 nonfermentative gram-negative bacilli, identified by conventional methods, were tested by the Crystal Enteric/Non-Fermenter ID kit and by the API 20E or API 20NE identification system. The overall correct results for fermenters were 92.9% by the Crystal kit and 89.1% by the API 20E system. The false identifications (genus and species incorrect) accounted for 3.1 and 7.1% for the Crystal and API systems, respectively. For nonfermenters, figures for correct identifications by the two systems were comparable (Crystal, 75.9%; API 20NE, 75.3%) while the API 20NE system gave twice as many incorrect results (13.8%) as Crystal (6.3%); however, Crystal failed to precisely identify several species included in a "miscellaneous" group. The Crystal Enteric/Non-Fermenter system is an easy-to-use kit which compares favorably with other commercial systems.
通过传统方法鉴定出的505株发酵型革兰氏阴性杆菌和201株非发酵型革兰氏阴性杆菌，采用Crystal肠道/非发酵菌鉴定试剂盒以及API 20E或API 20NE鉴定系统进行检测。对于发酵型细菌，Crystal试剂盒的总体正确结果为92.9%，API 20E系统为89.1%。Crystal和API系统的错误鉴定（属和种均错误）分别占3.1%和7.1%。对于非发酵型细菌，两种系统的正确鉴定数据相当（Crystal为75.9%；API 20NE为75.3%），而API 20NE系统的错误结果（13.8%）是Crystal（6.3%）的两倍；然而，Crystal未能准确鉴定出“其他”组中的几种细菌。Crystal肠道/非发酵菌系统是一种易于使用的试剂盒，与其他商业系统相比具有优势。
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