Peele D, Bradfield J, Pryor W, Vore S
Department of Comparative Medicine, East Carolina University School of Medicine, Greenville, North Carolina 27858, USA.
J Clin Microbiol. 1997 Jan;35(1):213-6. doi: 10.1128/jcm.35.1.213-216.1997.
This study compared the abilities of API 20E and BBL Crystal E/NF identification systems to correctly identify human and animal source gram-negative bacilli of known identifications, as provided by the American Type Culture Collection, Rockville, Md., and the Research Diagnostic and Investigative Laboratory, Columbia, Mo. Also addressed in the comparison are the cost, the relative ease of performing and interpreting the tests, and the potential problems surrounding each system. The two systems were comparable in terms of their respective costs and abilities to identify the bacteria tested. The cost per test was calculated as $4.69 for API 20E and $4.62 for Crystal E/NF. Of the animal source bacteria tested, Crystal E/NF identified 68% to the correct genus and species and 90% to the correct genus or group. The remaining 10% of the animal source bacteria were unidentified by Crystal. Human source bacteria tested by BBL Crystal E/NF gave very similar results: 47% correctly identified to genus and species, 90% correctly identified to genus or group, 7% unidentified, and 3% incorrectly identified. API 20E results were as follows for animal source bacteria: 53% correctly identified to genus and species, 76% correctly identified to genus or group, and 24% unidentified; the results for human source bacteria were as follows: 40% correctly identified to genus and species, 83% correctly identified to genus or group, and 17% unidentified. API 20E has a slightly more labor-intensive protocol for setting up the test than BBL Crystal E/NF but produced fewer questionable results.
本研究比较了API 20E鉴定系统和BBL Crystal E/NF鉴定系统对已知鉴定结果的人源和动物源革兰氏阴性杆菌的正确鉴定能力，这些菌株由位于马里兰州罗克维尔的美国模式培养物集存库以及位于密苏里州哥伦比亚的研究诊断与调查实验室提供。比较内容还包括成本、进行和解释测试的相对难易程度以及每个系统周围潜在的问题。在各自成本和鉴定所测试细菌的能力方面，这两个系统具有可比性。API 20E每次测试的成本计算为4.69美元，Crystal E/NF为4.62美元。在测试的动物源细菌中，Crystal E/NF将68%鉴定到正确的属和种，90%鉴定到正确的属或类群。Crystal未鉴定出其余10%的动物源细菌。BBL Crystal E/NF测试的人源细菌给出了非常相似的结果：47%正确鉴定到属和种，90%正确鉴定到属或类群，7%未鉴定，3%鉴定错误。API 20E对动物源细菌的结果如下：53%正确鉴定到属和种，76%正确鉴定到属或类群，24%未鉴定；对人源细菌的结果如下：40%正确鉴定到属和种，83%正确鉴定到属或类群，17%未鉴定。与BBL Crystal E/NF相比，API 20E设置测试的方案稍微更耗费人力，但产生的可疑结果较少。
