Bunny K L, Hall R M, Stokes H W
School of Biological Sciences, Macquarie University, Sydney, New South Wales, Australia.
Antimicrob Agents Chemother. 1995 Mar;39(3):686-93. doi: 10.1128/AAC.39.3.686.
The multidrug resistance plasmid pBWH301 was shown to contain a sull-associated integron with five inserted gene cassettes, aacA7-catB3-aadB-oxa2-orfD, all of which can be mobilized by the integron-encoded DNA integrase. The aadB, oxa2, and orfD cassettes are identical to known cassettes. The aacA7 gene encodes a protein that is a member of one of the three known families of aminoglycoside acetyltransferases classified as AAC(6')-I. The chloramphenicol acetyltransferase encoded by the catB3 gene is closely related to members of a recently identified family of chloramphenicol acetyltransferases. The catB3 gene displays a relatively high degree of sequence identity to a chromosomally located open reading frame in Pseudomonas aeruginosa, and this may represent evidence for the acquisition by a cassette of a chromosomal gene.
多药耐药质粒pBWH301被证明含有一个与sul相关的整合子，带有五个插入的基因盒，即aacA7-catB3-aadB-oxa2-orfD，所有这些基因盒都可被整合子编码的DNA整合酶所移动。aadB、oxa2和orfD基因盒与已知基因盒相同。aacA7基因编码一种蛋白质，它是被归类为AAC(6')-I的三个已知氨基糖苷类乙酰转移酶家族之一的成员。由catB3基因编码的氯霉素乙酰转移酶与最近鉴定出的一个氯霉素乙酰转移酶家族的成员密切相关。catB3基因与铜绿假单胞菌中一个位于染色体上的开放阅读框显示出相对较高的序列同一性，这可能代表了一个基因盒获取染色体基因的证据。
