Cohen Y, Perronne C, Lazard T, Truffot-Pernot C, Grosset J, Vilde J L, Pocidalo J J
Service des Maladies Infectieuses et Tropicales, Hôpital Raymond Poincaré, Garches, France.
Antimicrob Agents Chemother. 1995 Mar;39(3):735-8. doi: 10.1128/AAC.39.3.735.
Several murine models have been used to evaluate the activities of antimicrobial agents against Mycobacterium avium infection. The main model used is the beige mouse model, but beige mice are expensive and not easily available. Thus, we developed a model of infection in wild C57BL/6 mice. The drugs that exhibited some activity in a previous model of early infection were evaluated in a new model of established infection. Sparfloxacin (50 mg/kg of body weight), ethambutol (50 mg/kg), minocycline (25 mg/kg), and the inhibitor of the cortisol receptors RU-40555 (100 mg/kg) were compared with clarithromycin (50 mg/kg). Treatments were started 5 weeks after the inoculation and were continued for 21 days. Sparfloxacin and RU-40555, which exhibited a moderate activity in the model of early infection, were not effective in this model of established infection. Clarithromycin and combinations with clarithromycin kept their activities against M. avium infection, both in the spleen and in lungs. The present model of established infection of normal C57BL/6 mice is more relevant than the model of early infection for a stringent evaluation of drugs.
已有多种小鼠模型用于评估抗菌药物抗鸟分枝杆菌感染的活性。主要使用的模型是米色小鼠模型，但米色小鼠价格昂贵且不易获得。因此，我们建立了野生C57BL/6小鼠感染模型。在先前的早期感染模型中表现出一定活性的药物，在新建立的感染模型中进行了评估。将司帕沙星（50毫克/千克体重）、乙胺丁醇（50毫克/千克）、米诺环素（25毫克/千克）以及皮质醇受体抑制剂RU - 40555（100毫克/千克）与克拉霉素（50毫克/千克）进行比较。接种后5周开始治疗，并持续21天。在早期感染模型中表现出中等活性的司帕沙星和RU - 40555，在这个建立的感染模型中无效。克拉霉素及其联合用药在脾脏和肺部对鸟分枝杆菌感染均保持活性。对于严格评估药物而言，目前正常C57BL/6小鼠建立的感染模型比早期感染模型更具相关性。
