Lovley D R, Phillips E J, Lonergan D J, Widman P K
Appl Environ Microbiol. 1995 Jun;61(6):2132-8. doi: 10.1128/aem.61.6.2132-2138.1995.
There is a close phylogenetic relationship between Pelobacter species and members of the genera Desulfuromonas and Geobacter, and yet there has been a perplexing lack of physiological similarities. Pelobacter species have been considered to have a fermentative metabolism. In contrast, Desulfuromonas and Geobacter species have a respiratory metabolism with Fe(III) serving as the common terminal electron acceptor in all species. However, the ability of Pelobacter species to reduce Fe(III) had not been previously evaluated. When a culture of Pelobacter carbinolicus that had grown by fermentation of 2,3-butanediol was inoculated into the same medium supplemented with Fe(III), the Fe(III) was reduced. There was less accumulation of ethanol and more production of acetate in the presence of Fe(III). P. carbinolicus grew with ethanol as the sole electron donor and Fe(III) as the sole electron acceptor. Ethanol was metabolized to acetate. Growth was also possible on Fe(III) with the oxidation of propanol to propionate or butanol to butyrate if acetate was provided as a carbon source. P. carbinolicus appears capable of conserving energy to support growth from Fe(III) respiration as it also grew with H2 or formate as the electron donor and Fe(III) as the electron acceptor. Once adapted to Fe(III) reduction, P. carbinolicus could also grow on ethanol or H2 with S0 as the electron acceptor. P. carbinolicus did not contain detectable concentrations of the c-type cytochromes that previous studies have suggested are involved in electron transport to Fe(III) in other organisms that conserve energy to support growth from Fe(III) reduction.(ABSTRACT TRUNCATED AT 250 WORDS)
佩洛杆菌属物种与脱硫单胞菌属和地杆菌属的成员之间存在密切的系统发育关系，但令人困惑的是它们在生理上缺乏相似性。佩洛杆菌属物种被认为具有发酵代谢。相比之下，脱硫单胞菌属和地杆菌属物种具有呼吸代谢，在所有物种中均以Fe(III)作为共同的末端电子受体。然而，此前尚未评估过佩洛杆菌属物种还原Fe(III)的能力。当将通过2,3 - 丁二醇发酵生长的乙醇氧化佩洛杆菌培养物接种到添加了Fe(III)的相同培养基中时，Fe(III)被还原了。在有Fe(III)存在的情况下，乙醇的积累减少，乙酸的产生增加。乙醇氧化佩洛杆菌能够以乙醇作为唯一电子供体、Fe(III)作为唯一电子受体生长。乙醇被代谢为乙酸。如果提供乙酸作为碳源，在Fe(III)上以丙醇氧化为丙酸或丁醇氧化为丁酸的方式生长也是可能的。乙醇氧化佩洛杆菌似乎能够通过Fe(III)呼吸来保存能量以支持生长，因为它也能以H2或甲酸作为电子供体、Fe(III)作为电子受体生长。一旦适应了Fe(III)还原，乙醇氧化佩洛杆菌也能以乙醇或H2作为电子供体、S0作为电子受体生长。乙醇氧化佩洛杆菌中未检测到可检测浓度的c型细胞色素，而此前的研究表明，在其他通过Fe(III)还原保存能量以支持生长的生物体中，这些c型细胞色素参与电子传递至Fe(III)。（摘要截选至250字）
