Ronca F, Palmieri L, Malengo S, Bertelli A
Institute of Biological Chemistry, University of Pisa, Italy.
Int J Tissue React. 1994;16(4):187-94.
Sickle-cell anaemia erythrocytes are under oxidative stress which contributes to some of the reversible and irreversible modifications observed in these cells. L-Propionyl carnitine, which protects myocardium, endothelium and erythrocytes from peroxidative damages and is able to stabilize damaged cell membranes, is also able to decrease the formation of thiobarbituric acid reactive substances which are produced by incubating erythrocytes with hydrogen peroxide in the presence of atmospheric oxygen or 95% N2-5% CO2 mixture. In these experimental conditions the increase of thiobarbituric-acid-reactive substances is significantly lower at 5 mM and 10 mM L-propionyl-carnitine concentrations. The formation of irreversibly sickled cells induced by 24-h incubation of sickle-cell anaemia erythrocytes under 95% N2-5% CO2 mixture is significantly decreased in the presence of 1 mM or higher L-propionyl-carnitine concentrations. The percent filtration of sickle red blood cells through micropore filters is significantly decreased at oxygen tensions between 20 and 40 mmHg. These in-vitro observations suggest that L-propionyl carnitine may be beneficial in maintaining the normal shape of sickle-cell anaemia erythrocytes at low oxygen tension and in decreasing the peroxidative damages which accumulate during the life of red blood cells.
镰状细胞贫血红细胞处于氧化应激状态，这导致了在这些细胞中观察到的一些可逆和不可逆的改变。L-丙酰肉碱可保护心肌、内皮细胞和红细胞免受过氧化损伤，并能够稳定受损的细胞膜，它还能够减少硫代巴比妥酸反应性物质的形成，这些物质是在大气氧或95% N2-5% CO2混合物存在的情况下，将红细胞与过氧化氢孵育产生的。在这些实验条件下，在5 mM和10 mM L-丙酰肉碱浓度下，硫代巴比妥酸反应性物质的增加显著降低。在95% N2-5% CO2混合物下，将镰状细胞贫血红细胞孵育24小时诱导产生的不可逆镰状细胞的形成，在1 mM或更高的L-丙酰肉碱浓度存在时显著减少。在20至40 mmHg的氧张力下，镰状红细胞通过微孔滤器的过滤百分比显著降低。这些体外观察结果表明，L-丙酰肉碱可能有助于在低氧张力下维持镰状细胞贫血红细胞的正常形状，并减少红细胞寿命期间积累的过氧化损伤。
