Akasawa A, Hsieh L S, Lin Y
Laboratory of Immunobiochemistry, Food and Drug Administration, Rockville, MD 20852-1441, USA.
J Allergy Clin Immunol. 1995 Jun;95(6):1196-205. doi: 10.1016/s0091-6749(95)70076-5.
In recent years there has been an increasing incidence of allergy to latex among health care workers and children with spina bifida. The allergic response in these individuals can be severe and occasionally fatal. Several allergens have been identified with the use of sera from different patient groups. In our effort to identify reagents for in vitro testing and clinical use, we investigated the reactivities of latex proteins to sera collected from a wide range of patients with latex allergy.
Twenty-six serum samples were obtained from adult patients with latex allergy, both hospital workers and non-hospital workers. Serum pools were made either from sera of children with spina bifida or sera of adult patients with latex allergy. Proteins from C-serum and latex particles of latex sap (nonammoniated) were separated by different gel electrophoresis techniques and evaluated for specific IgE binding by immunoblotting.
More than 50% of the sera tested reacted to an 18 kd protein, a 25.6 kd acidic protein with an isoelectric point of 3.5, or to both proteins; whereas only 23% of the individual serum samples tested reacted to the rubber elongation factor, which has been reported to be a major latex allergen. The immunoreactive patterns of children's and adults' serum pools were similar but not identical.
With the use of gel electrophoresis and immunoblotting techniques, different immunoreactive proteins were identified in C-serum and particles of latex. Rubber elongation factor, which reacted to only 23% of sera tested, did not appear to cross-react immunologically with other latex allergens.
近年来，医护人员和脊柱裂儿童对乳胶过敏的发生率不断上升。这些个体的过敏反应可能很严重，偶尔甚至会致命。通过使用来自不同患者群体的血清，已鉴定出几种过敏原。为了鉴定用于体外检测和临床使用的试剂，我们研究了乳胶蛋白与从广泛的乳胶过敏患者收集的血清之间的反应性。
从乳胶过敏的成年患者（包括医院工作人员和非医院工作人员）中获取了26份血清样本。血清库由脊柱裂儿童的血清或乳胶过敏成年患者的血清制成。通过不同的凝胶电泳技术分离来自C血清和乳胶汁液（未氨化）的乳胶颗粒中的蛋白质，并通过免疫印迹评估特异性IgE结合。
超过50%的测试血清与一种18kd的蛋白质、一种等电点为3.5的25.6kd酸性蛋白质或这两种蛋白质都发生反应；而在测试的个体血清样本中，只有23%与橡胶延伸因子发生反应，据报道橡胶延伸因子是一种主要的乳胶过敏原。儿童和成人血清库的免疫反应模式相似但不完全相同。
通过使用凝胶电泳和免疫印迹技术，在C血清和乳胶颗粒中鉴定出了不同的免疫反应性蛋白质。仅与23%的测试血清发生反应的橡胶延伸因子，似乎在免疫学上与其他乳胶过敏原没有交叉反应。
