Gagnon R, Boutin Y, Hébert J
Centre de Recherche en Immunologie et Allergie, Ste-Foy, Quebec, Canada.
J Allergy Clin Immunol. 1995 Jun;95(6):1268-75. doi: 10.1016/s0091-6749(95)70085-4.
The development of an animal model representative of the in vivo situation of human atopic diseases is always of interest for a better understanding of IgE production and regulation. Along these lines, mice with severe combined immunodeficiency (SCID mice) engrafted with lymphocytes from atopic subjects might be a suitable model for such studies.
This study aims to analyze the production of Lol p I-specific IgE and IgG antibodies in SCID mice after transplantation of human peripheral blood mononuclear cells from atopic patients sensitive to grass pollens and from nonatopic donors.
Peripheral blood mononuclear cells were transplanted into SCID mice, which were then challenged with Lol p I, and antibody responses (IgG and IgE) were analyzed over a 6-week period.
Total IgG antibody was measured in each mouse serum after transplantation. Also, most mice (regardless of whether donors were atopic) that were challenged with Lol p I produced specific IgG antibody. Total IgE antibody production was observed only in mice grafted with cells from atopic patients. Lol p I-specific IgE antibodies were also produced after immunization with Lol p I. Although IgG antibody/response tended to plateau, the IgE antibody response increased until it peaked and declined thereafter. Interferon-gamma was detected in sera from mice producing IgE antibody, which supports a possible role of interferon-gamma in the decrease of IgE response.
This study suggests that the SCID mouse model could represent an interesting approach to studying specific, total IgG and IgE antibody production, and ultimately their regulation.
开发一种能够代表人类特应性疾病体内情况的动物模型，对于更好地理解IgE的产生和调节一直具有重要意义。按照这一思路，移植了特应性个体淋巴细胞的严重联合免疫缺陷小鼠（SCID小鼠）可能是此类研究的合适模型。
本研究旨在分析将对草花粉敏感的特应性患者和非特应性供体的人外周血单个核细胞移植到SCID小鼠后，SCID小鼠中Lol p I特异性IgE和IgG抗体的产生情况。
将外周血单个核细胞移植到SCID小鼠体内，然后用Lol p I进行攻击，并在6周内分析抗体反应（IgG和IgE）。
移植后测定每只小鼠血清中的总IgG抗体。此外，大多数用Lol p I攻击的小鼠（无论供体是否为特应性）都产生了特异性IgG抗体。仅在移植了特应性患者细胞的小鼠中观察到总IgE抗体的产生。用Lol p I免疫后也产生了Lol p I特异性IgE抗体。尽管IgG抗体反应趋于平稳，但IgE抗体反应持续增加，直至达到峰值，随后下降。在产生IgE抗体的小鼠血清中检测到干扰素-γ，这支持了干扰素-γ在降低IgE反应中可能发挥的作用。
本研究表明，SCID小鼠模型可能是研究特异性、总IgG和IgE抗体产生及其最终调节的一种有趣方法。
