Evins G M, Cameron D N, Wells J G, Greene K D, Popovic T, Giono-Cerezo S, Wachsmuth I K, Tauxe R V
Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USA.
J Infect Dis. 1995 Jul;172(1):173-9. doi: 10.1093/infdis/172.1.173.
Since the Latin American cholera epidemic began in 1991, 447 isolates of Vibrio cholerae O1 from the Western Hemisphere have been assayed by multilocus enzyme electrophoresis (MEE) to determine allelic variation among 16 enzyme-encoding genes. Two electrophoretic types (ETs) were identified among toxigenic isolates from Latin America: 323 were ET 4, the ET associated with the Latin American epidemic, and 29 were ET 3. Twenty-three of these ET 3 isolates had a distinctive antimicrobial resistance pattern also seen in isolates imported into the United States from Latin America and Southeast Asia. These resistant isolates had an identical ribotype and nearly identical pulsed-field gel electrophoresis (PFGE) patterns. Most nontoxigenic isolates analyzed were not precursors or descendants of toxigenic epidemic strains. MEE provided a population genetic frame-work for the interpretation of PFGE and ribotype data from the isolates in this study. All three methods identified 2 distinct strains of toxigenic V. cholerae O1 currently epidemic in Latin America.
自1991年拉丁美洲霍乱疫情开始以来，已通过多位点酶电泳（MEE）对来自西半球的447株霍乱弧菌O1分离株进行了分析，以确定16个编码酶的基因中的等位基因变异。在来自拉丁美洲的产毒分离株中鉴定出两种电泳类型（ETs）：323株为ET 4，即与拉丁美洲疫情相关的电泳类型，29株为ET 3。这些ET 3分离株中有23株具有独特的抗菌耐药模式，从拉丁美洲和东南亚进口到美国的分离株中也可见到这种模式。这些耐药分离株具有相同的核糖体型和几乎相同的脉冲场凝胶电泳（PFGE）模式。分析的大多数非产毒分离株不是产毒流行菌株的前体或后代。MEE为解释本研究中分离株的PFGE和核糖体型数据提供了群体遗传学框架。所有三种方法都鉴定出目前在拉丁美洲流行的2种不同的产毒霍乱弧菌O1菌株。
