Groen J, Gerding M, Koeman J P, Roholl P J, van Amerongen G, Jordans H G, Niesters H G, Osterhaus A D
Department of Clinical Virology, University Hospital Rotterdam, Netherlands.
J Infect Dis. 1995 Jul;172(1):38-44. doi: 10.1093/infdis/172.1.38.
Cynomolgus macaques (Macaca fascicularis) were experimentally infected with Puumala virus (strain Hällnäs), which causes nephropathia epidemica in humans in western Europe. During the first week after intratracheal inoculation, the monkeys exhibited signs of lethargy followed by mild proteinuria and microhematuria. Histopathologic changes during the first 7 weeks after infection were largely confined to abnormalities in medullary tubular cells of the kidneys, which coincided with the demonstration of viral antigen and viral RNA. The development of different classes of virus-specific plasma antibodies to the respective viral antigens were similar to those observed in humans with nephropathia epidemica. This first description of a nonhuman primate model for hantavirus infection shows that the cynomolgus macaque provides a suitable model with which to study the pathogenesis of Puumala virus infections and to evaluate new diagnostic methods, immunization strategies, and therapies.
食蟹猴（猕猴属）被实验性感染普马拉病毒（哈尔纳斯毒株），该病毒在西欧可导致人类患流行性肾病。气管内接种后的第一周，猴子表现出嗜睡迹象，随后出现轻度蛋白尿和微量血尿。感染后前7周的组织病理学变化主要局限于肾脏髓质肾小管细胞的异常，这与病毒抗原和病毒RNA的检测结果相符。针对相应病毒抗原产生的不同类别的病毒特异性血浆抗体的发展情况与流行性肾病患者中观察到的情况相似。对汉坦病毒感染的非人灵长类动物模型的这一首次描述表明，食蟹猴为研究普马拉病毒感染的发病机制以及评估新的诊断方法、免疫策略和治疗方法提供了一个合适的模型。
