枯草芽孢杆菌中存在两个编码尿嘧啶磷酸核糖转移酶的基因。
Two genes encoding uracil phosphoribosyltransferase are present in Bacillus subtilis.
作者信息
Martinussen J, Glaser P, Andersen P S, Saxild H H
机构信息
Center for Lactic Acid Bacteria, Technical University of Denmark, Lyngby.
出版信息
J Bacteriol. 1995 Jan;177(1):271-4. doi: 10.1128/jb.177.1.271-274.1995.
Abstract
Uracil phosphoribosyltransferase (UPRTase) catalyzes the key reaction in the salvage of uracil in many microorganisms. Surprisingly, two genes encoding UPRTase activity were cloned from Bacillus subtilis by complementation of an Escherichia coli mutant. The genes were sequenced, and the putative amino acid sequences were deduced. One gene showed a high level of homology to UPRTases from other organisms, whereas the other gene with a low level of homology to other UPRTases turned out to be the pyrR gene--the repressor of the pyr operon. The role of these genes in uracil metabolism was established by an analysis of the phenotypes of upp and pyrR mutants.
摘要
尿嘧啶磷酸核糖转移酶（UPRTase）催化许多微生物中尿嘧啶补救途径的关键反应。令人惊讶的是，通过互补大肠杆菌突变体，从枯草芽孢杆菌中克隆出了两个编码UPRTase活性的基因。对这些基因进行了测序，并推导了推定的氨基酸序列。其中一个基因与其他生物的UPRTase具有高度同源性，而另一个与其他UPRTase同源性较低的基因原来是pyrR基因——嘧啶操纵子的阻遏物。通过分析upp和pyrR突变体的表型，确定了这些基因在尿嘧啶代谢中的作用。
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