• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Two genes encoding uracil phosphoribosyltransferase are present in Bacillus subtilis.枯草芽孢杆菌中存在两个编码尿嘧啶磷酸核糖转移酶的基因。
J Bacteriol. 1995 Jan;177(1):271-4. doi: 10.1128/jb.177.1.271-274.1995.
2
Mutations in Bacillus subtilis PyrR, the pyr regulatory protein, with defects in regulation by pyrimidines.枯草芽孢杆菌嘧啶调节蛋白PyrR中的突变，嘧啶调节存在缺陷。
FEMS Microbiol Lett. 1996 Mar 15;137(1):13-8. doi: 10.1111/j.1574-6968.1996.tb08075.x.
3
The pyrimidine biosynthesis operon of the thermophile Bacillus caldolyticus includes genes for uracil phosphoribosyltransferase and uracil permease.嗜热栖热芽孢杆菌的嘧啶生物合成操纵子包含尿嘧啶磷酸核糖基转移酶和尿嘧啶通透酶的基因。
J Bacteriol. 1994 Jun;176(12):3698-707. doi: 10.1128/jb.176.12.3698-3707.1994.
4
Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism.枯草芽孢杆菌嘧啶生物合成（pyr）基因簇通过自体转录衰减机制进行调控。
J Bacteriol. 1994 Jun;176(12):3708-22. doi: 10.1128/jb.176.12.3708-3722.1994.
5
Cloning and characterization of upp, a gene encoding uracil phosphoribosyltransferase from Lactococcus lactis.来自乳酸乳球菌的尿嘧啶磷酸核糖转移酶编码基因upp的克隆与鉴定。
J Bacteriol. 1994 Nov;176(21):6457-63. doi: 10.1128/jb.176.21.6457-6463.1994.
6
Purification and characterization of Bacillus subtilis PyrR, a bifunctional pyr mRNA-binding attenuation protein/uracil phosphoribosyltransferase.枯草芽孢杆菌双功能pyr mRNA结合衰减蛋白/尿嘧啶磷酸核糖转移酶PyrR的纯化与特性分析
J Biol Chem. 1998 Mar 6;273(10):5932-8. doi: 10.1074/jbc.273.10.5932.
7
Adaptation of an enzyme to regulatory function: structure of Bacillus subtilis PyrR, a pyr RNA-binding attenuation protein and uracil phosphoribosyltransferase.酶对调节功能的适应性：枯草芽孢杆菌PyrR的结构，一种pyr RNA结合衰减蛋白和尿嘧啶磷酸核糖转移酶。
Structure. 1998 Mar 15;6(3):337-50. doi: 10.1016/s0969-2126(98)00036-7.
8
Kinetic studies of the uracil phosphoribosyltransferase reaction catalyzed by the Bacillus subtilis pyrimidine attenuation regulatory protein PyrR.枯草芽孢杆菌嘧啶衰减调节蛋白PyrR催化尿嘧啶磷酸核糖基转移酶反应的动力学研究。
J Biol Chem. 2003 Feb 28;278(9):6921-7. doi: 10.1074/jbc.M211111200. Epub 2002 Dec 13.
9
Characterization of the interaction of Bacillus subtilis PyrR with pyr mRNA by site-directed mutagenesis of the protein.通过对蛋白质进行定点诱变来表征枯草芽孢杆菌PyrR与pyr mRNA的相互作用。
J Bacteriol. 2002 May;184(9):2521-8. doi: 10.1128/JB.184.9.2521-2528.2002.
10
Transcriptional attenuation of the Bacillus subtilis pyr operon by the PyrR regulatory protein and uridine nucleotides in vitro.枯草芽孢杆菌pyr操纵子在体外受PyrR调节蛋白和尿苷核苷酸的转录衰减作用。
J Bacteriol. 1996 Dec;178(24):7206-11. doi: 10.1128/jb.178.24.7206-7211.1996.

引用本文的文献

1
Regulation of Bacterial Gene Expression by Transcription Attenuation.转录衰减调控细菌基因表达。
Microbiol Mol Biol Rev. 2019 Jul 3;83(3). doi: 10.1128/MMBR.00019-19. Print 2019 Aug 21.
2
Phosphoribosyl Diphosphate (PRPP): Biosynthesis, Enzymology, Utilization, and Metabolic Significance.磷酸核糖焦磷酸（PRPP）：生物合成、酶学、利用及代谢意义
Microbiol Mol Biol Rev. 2016 Dec 28;81(1). doi: 10.1128/MMBR.00040-16. Print 2017 Mar.
3
Metabolic and genetic factors affecting the productivity of pyrimidine nucleoside in Bacillus subtilis.影响枯草芽孢杆菌中嘧啶核苷产量的代谢和遗传因素。
Microb Cell Fact. 2015 Apr 15;14:54. doi: 10.1186/s12934-015-0237-1.
4
Development and application of a upp-based counterselective gene replacement system for the study of the S-layer protein SlpX of Lactobacillus acidophilus NCFM.基于upp的反选择基因替换系统的开发与应用，用于嗜酸乳杆菌NCFM表面层蛋白SlpX的研究
Appl Environ Microbiol. 2009 May;75(10):3093-105. doi: 10.1128/AEM.02502-08. Epub 2009 Mar 20.
5
Regulation of pyrimidine biosynthetic gene expression in bacteria: repression without repressors.细菌中嘧啶生物合成基因表达的调控：无阻遏物的阻遏作用
Microbiol Mol Biol Rev. 2008 Jun;72(2):266-300, table of contents. doi: 10.1128/MMBR.00001-08.
6
Identification and characterization of human uracil phosphoribosyltransferase (UPRTase).人尿嘧啶磷酸核糖转移酶（UPRTase）的鉴定与特性分析
J Hum Genet. 2007;52(5):415-422. doi: 10.1007/s10038-007-0129-2. Epub 2007 Mar 24.
7
Uracil salvage pathway in Lactobacillus plantarum: Transcription and genetic studies.植物乳杆菌中的尿嘧啶补救途径：转录与遗传学研究。
J Bacteriol. 2006 Jul;188(13):4777-86. doi: 10.1128/JB.00195-06.
8
Repression of the pyr operon in Lactobacillus plantarum prevents its ability to grow at low carbon dioxide levels.植物乳杆菌中pyr操纵子的抑制作用会阻碍其在低二氧化碳水平下生长的能力。
J Bacteriol. 2005 Mar;187(6):2093-104. doi: 10.1128/JB.187.6.2093-2104.2005.
9
Structure of the nucleotide complex of PyrR, the pyr attenuation protein from Bacillus caldolyticus, suggests dual regulation by pyrimidine and purine nucleotides.嗜热解芽孢杆菌的嘧啶衰减蛋白PyrR核苷酸复合物的结构表明其受嘧啶和嘌呤核苷酸的双重调控。
J Bacteriol. 2005 Mar;187(5):1773-82. doi: 10.1128/JB.187.5.1773-1782.2005.
10
Regulation of transcription of the Bacillus subtilis pyrG gene, encoding cytidine triphosphate synthetase.枯草芽孢杆菌中编码胞苷三磷酸合成酶的pyrG基因转录的调控
J Bacteriol. 2001 Oct;183(19):5513-22. doi: 10.1128/JB.183.19.5513-5522.2001.

本文引用的文献

1
TRANSFORMATION OF BIOCHEMICALLY DEFICIENT STRAINS OF BACILLUS SUBTILIS BY DEOXYRIBONUCLEATE.脱氧核糖核酸对枯草芽孢杆菌生化缺陷菌株的转化
Proc Natl Acad Sci U S A. 1958 Oct 15;44(10):1072-8. doi: 10.1073/pnas.44.10.1072.
2
The ftf gene encoding the cell-bound fructosyltransferase of Streptococcus salivarius ATCC 25975 is preceded by an insertion sequence and followed by FUR1 and clpP homologues.编码唾液链球菌ATCC 25975细胞结合型果糖基转移酶的ftf基因之前有一个插入序列，之后是FUR1和clpP同源物。
J Gen Microbiol. 1993 May;139(5):913-20. doi: 10.1099/00221287-139-5-913.
3
Regulation of the Bacillus subtilis pyrimidine biosynthetic (pyr) gene cluster by an autogenous transcriptional attenuation mechanism.枯草芽孢杆菌嘧啶生物合成（pyr）基因簇通过自体转录衰减机制进行调控。
J Bacteriol. 1994 Jun;176(12):3708-22. doi: 10.1128/jb.176.12.3708-3722.1994.
4
An improved positive selection plasmid vector constructed by oligonucleotide mediated mutagenesis.通过寡核苷酸介导诱变构建的改良型阳性选择质粒载体。
Nucleic Acids Res. 1983 Nov 25;11(22):8019-30. doi: 10.1093/nar/11.22.8019.
5
Mutations affecting uridine monophosphate pyrophosphorylase or the argR gene in Escherichia coli. Effects on carbamoyl phosphate and pyrimidine biosynthesis and on uracil uptake.影响大肠杆菌中尿苷单磷酸焦磷酸化酶或argR基因的突变。对氨甲酰磷酸和嘧啶生物合成以及尿嘧啶摄取的影响。
Mol Gen Genet. 1972;118(3):235-45. doi: 10.1007/BF00333460.
6
Purification and some properties of uracil phosphoribosyltransferase from Escherichia coli K12.来自大肠杆菌K12的尿嘧啶磷酸核糖基转移酶的纯化及某些性质
Biochim Biophys Acta. 1986 Apr 11;881(2):268-75. doi: 10.1016/0304-4165(86)90013-9.
7
Genetic and physiological characterization of Bacillus subtilis mutants resistant to purine analogs.对嘌呤类似物具有抗性的枯草芽孢杆菌突变体的遗传和生理学特征分析。
J Bacteriol. 1987 Jul;169(7):2977-83. doi: 10.1128/jb.169.7.2977-2983.1987.
8
Functional organization and nucleotide sequence of the Bacillus subtilis pyrimidine biosynthetic operon.枯草芽孢杆菌嘧啶生物合成操纵子的功能组织与核苷酸序列。
J Biol Chem. 1991 May 15;266(14):9113-27.
9
Characterization of the upp gene encoding uracil phosphoribosyltransferase of Escherichia coli K12.大肠杆菌K12中编码尿嘧啶磷酸核糖转移酶的upp基因的特性分析。
Eur J Biochem. 1992 Feb 15;204(1):51-6. doi: 10.1111/j.1432-1033.1992.tb16604.x.
10
DNA sequencing with chain-terminating inhibitors.使用链终止抑制剂的DNA测序。
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7. doi: 10.1073/pnas.74.12.5463.

枯草芽孢杆菌中存在两个编码尿嘧啶磷酸核糖转移酶的基因。

Two genes encoding uracil phosphoribosyltransferase are present in Bacillus subtilis.

作者信息

Martinussen J, Glaser P, Andersen P S, Saxild H H

机构信息

Center for Lactic Acid Bacteria, Technical University of Denmark, Lyngby.

出版信息

J Bacteriol. 1995 Jan;177(1):271-4. doi: 10.1128/jb.177.1.271-274.1995.

DOI:10.1128/jb.177.1.271-274.1995
PMID:7798145
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC176586/
Abstract

Uracil phosphoribosyltransferase (UPRTase) catalyzes the key reaction in the salvage of uracil in many microorganisms. Surprisingly, two genes encoding UPRTase activity were cloned from Bacillus subtilis by complementation of an Escherichia coli mutant. The genes were sequenced, and the putative amino acid sequences were deduced. One gene showed a high level of homology to UPRTases from other organisms, whereas the other gene with a low level of homology to other UPRTases turned out to be the pyrR gene--the repressor of the pyr operon. The role of these genes in uracil metabolism was established by an analysis of the phenotypes of upp and pyrR mutants.

摘要

尿嘧啶磷酸核糖转移酶（UPRTase）催化许多微生物中尿嘧啶补救途径的关键反应。令人惊讶的是，通过互补大肠杆菌突变体，从枯草芽孢杆菌中克隆出了两个编码UPRTase活性的基因。对这些基因进行了测序，并推导了推定的氨基酸序列。其中一个基因与其他生物的UPRTase具有高度同源性，而另一个与其他UPRTase同源性较低的基因原来是pyrR基因——嘧啶操纵子的阻遏物。通过分析upp和pyrR突变体的表型，确定了这些基因在尿嘧啶代谢中的作用。