DePaoli A M, Hurley K M, Yasada K, Reisine T, Bell G
Howard Hughes Medical Institute, University of Chicago, Illinois 60637.
Mol Cell Neurosci. 1994 Aug;5(4):327-35. doi: 10.1006/mcne.1994.1039.
The distribution of the kappa opioid receptor mRNA in adult mouse brain has been determined using the technique of in situ hybridization histochemistry. The mRNA for the kappa opioid receptor was expressed in distinct areas throughout the brain. The telencephalon showed high levels of expression in the deeper layers of the parietal and temporal cortex, olfactory tubercle, nucleus accumbens, claustrum, endopiriform nucleus, nucleus of the vertical and horizontal limb of the diagonal band, and medial and central nuclei of the amygdala. In the diencephalon, kappa opioid receptor mRNA was present in multiple medial thalamic nuclei including the centromedial, paraventricular, parafasicular, central, and peritenial nuclei, as well as in most hypothalamic nuclei including the ventromedial, periventricular, supraoptic, arcuate, and dorsomedial nuclei. The mesencephalon showed highest levels of kappa receptor mRNA in the substantia nigra pars compacta, ventral tegmental area, zona incerta, interpeduncular nucleus, superior colliculus, inferior colliculus, central grey, and the raphe nucleus. In the metencephalon, kappa opioid receptor mRNA was expressed in the parabrachial nuclei, locus coeruleus, dorsal and ventral tegmental nuclei, and the raphe pontine nuclei. The distribution of the kappa receptor mRNA closely coincides with the localization of binding sites in rat brain for [3H]U-69,593, a specific kappa 1 opioid receptor ligand. The mRNA distribution also correlates with neuroanatomical sites of actions of kappa agonists and distribution of the endogenous kappa receptor ligand dynorphin.
利用原位杂交组织化学技术已确定成年小鼠脑中κ阿片受体mRNA的分布。κ阿片受体的mRNA在整个大脑的不同区域表达。端脑在顶叶和颞叶皮质深层、嗅结节、伏隔核、屏状核、内梨状核、斜角带垂直和水平支核以及杏仁核的内侧和中央核中显示出高水平表达。在间脑，κ阿片受体mRNA存在于多个内侧丘脑核，包括中央内侧核、室旁核、束旁核、中央核和终板旁核以及大多数下丘脑核，包括腹内侧核、室周核、视上核、弓状核和背内侧核。中脑在黑质致密部、腹侧被盖区、未定带、脚间核、上丘、下丘、中央灰质和中缝核中显示出最高水平的κ受体mRNA。在脑桥，κ阿片受体mRNA在臂旁核、蓝斑、背侧和腹侧被盖核以及脑桥中缝核中表达。κ受体mRNA的分布与大鼠脑中特异性κ1阿片受体配体[3H]U-69,593结合位点的定位密切吻合。mRNA的分布也与κ激动剂的神经解剖作用部位以及内源性κ受体配体强啡肽的分布相关。
