Watson J B, Coulter P M, Margulies J E, de Lecea L, Danielson P E, Erlander M G, Sutcliffe J G
Department of Psychiatry and Biobehavioral Sciences, UCLA School of Medicine.
J Neurosci Res. 1994 Sep 1;39(1):108-16. doi: 10.1002/jnr.490390113.
We used subtractive hybridization to isolate clones of gamma 7, a 68 residue G-protein gamma subunit. Northern blotting and in situ hybridization reveal that the gamma 7 subunit mRNA is expressed primarily in medium-sized neurons of the neostriatum and nucleus accumbens and neurons of the olfactory tubercle, and at low levels in the dentate gyrus of the hippocampal formation and laminae II-III, and V of the neocortex. The gamma 7 mRNA is translocated into dendrites of neurons in the neostriatum and the dentate gyrus of the hippocampus. gamma 7 is expressed at relatively very low concentrations in peripheral tissues. The selective pattern of gamma 7 expression within the brain is highly reminiscent of those of the striatum-enriched adenylyl cyclase ACST, dopamine receptors, and the alpha subunit of G(olf), suggesting that, in striatum, gamma 7 may be a subunit of a G(olf) alpha-containing G protein that couples dopamine receptors selectively to ACST.
我们利用消减杂交技术分离出γ7的克隆体，γ7是一种含68个氨基酸残基的G蛋白γ亚基。Northern印迹法和原位杂交显示，γ7亚基mRNA主要在新纹状体和伏隔核的中等大小神经元以及嗅结节的神经元中表达，在海马结构的齿状回以及新皮质的II - III层和V层中表达水平较低。γ7 mRNA被转运到新纹状体和海马齿状回神经元的树突中。γ7在外周组织中的表达浓度相对非常低。γ7在脑内的选择性表达模式与富含纹状体的腺苷酸环化酶ACST、多巴胺受体以及G(olf)的α亚基的表达模式高度相似，这表明在纹状体中，γ7可能是一种含G(olf)α的G蛋白的亚基，该G蛋白将多巴胺受体选择性地与ACST偶联。
