Moussy F, Harrison D J, O'Brien D W, Rajotte R V
Department of Chemistry, University of Alberta, Edmonton, Canada.
Anal Chem. 1993 Aug 1;65(15):2072-7. doi: 10.1021/ac00063a023.
A miniature, needle-type glucose sensor based on a new trilayer membrane configuration has been prepared and evaluated both in vitro and in vivo. The perfluorinated ionomer, Nafion, was used as a protective, biocompatible, outer coating, and poly(o-phenylenediamine) as an inner coating to reduce interference by small, electroactive compounds. Glucose oxidase immobilized in a bovine serum albumin matrix was sandwiched between these coatings. The entire sensor assembly of Pt working electrode and Ag/AgCl reference electrode was 0.5 mm in diameter and could be inserted subcutaneously through an 18-gauge needle. The sensor current closely followed the plasma glucose level during a glucose tolerance test in active dogs, with a delay of 3 min, corresponding to the known lag time for subcutaneous glucose levels. The sensor remained functional after 1 week of implantation, but failed after 2 weeks due to degradation of the reference electrode. In vitro tests in pH 7.4 buffer or whole blood show the sensors have good selectivity, sensitivity of about 25 nA/mM, precision of 2-5%, and a 90% response time of 33 s. Stabilization following polarization requires 10-30 min in vitro and 30-40 min in vivo.
一种基于新型三层膜结构的微型针型葡萄糖传感器已制备完成，并进行了体外和体内评估。全氟离子聚合物Nafion用作保护性生物相容性外涂层，聚邻苯二胺用作内涂层以减少小型电活性化合物的干扰。固定在牛血清白蛋白基质中的葡萄糖氧化酶夹在这些涂层之间。整个由铂工作电极和银/氯化银参比电极组成的传感器组件直径为0.5毫米，可通过18号针头皮下插入。在活跃犬的葡萄糖耐量试验期间，传感器电流紧密跟随血浆葡萄糖水平，延迟3分钟，这与皮下葡萄糖水平已知的滞后时间相对应。植入1周后传感器仍能正常工作，但2周后由于参比电极降解而失效。在pH 7.4缓冲液或全血中的体外测试表明，该传感器具有良好的选择性、约25 nA/mM的灵敏度、2 - 5%的精密度以及90%响应时间为33秒。极化后的稳定在体外需要10 - 30分钟，在体内需要30 - 40分钟。
