通过聚合酶链反应（PCR）及直接DNA测序对粪肠球菌中与氟喹诺酮耐药性相关的gyrA基因突变进行分析。
Analysis by PCR and direct DNA sequencing of gyrA mutations associated with fluoroquinolone resistance in Enterococcus faecalis.
作者信息
Korten V, Huang W M, Murray B E
机构信息
Center for Infectious Diseases, University of Texas Medical School, Houston 77030.
出版信息
Antimicrob Agents Chemother. 1994 Sep;38(9):2091-4. doi: 10.1128/AAC.38.9.2091.
Abstract
A region of gyrA, the gene encoding subunit A of DNA gyrase, that is known to be associated with resistance was amplified and sequenced from 16 Enterococcus faecalis and Enterococcus faecium isolates. Six ciprofloxacin-resistant clinical isolates (MICs of ciprofloxacin, 32 to 64 micrograms/ml) and one multistep resistant laboratory mutant of E. faecalis (MIC of ciprofloxacin, 128 micrograms/ml) contained a change from serine to arginine or to isoleucine at codon 83 or a change from glutamic acid to lysine or to glycine at codon 87 (Escherichia coli GyrA coordinates); these changes have been associated with fluoroquinolone resistance in other species. No difference in the region studied was found in two ciprofloxacin-resistant E. faecium isolates (MICs, 32 micrograms/ml) or in four laboratory derived, spontaneous ciprofloxacin-resistant mutants of E. faecalis (MICs, 8 to 16 micrograms/ml), suggesting that other mechanisms may be responsible for fluoroquinolone resistance in some enterococci.
摘要
从16株粪肠球菌和屎肠球菌分离株中扩增并测序了gyrA基因的一个区域，该基因编码DNA促旋酶的A亚基，已知该区域与耐药性相关。6株对环丙沙星耐药的临床分离株（环丙沙星的最低抑菌浓度为32至64微克/毫升）和1株粪肠球菌的多步耐药实验室突变株（环丙沙星的最低抑菌浓度为128微克/毫升）在第83位密码子处发生了从丝氨酸到精氨酸或异亮氨酸的变化，或在第87位密码子处发生了从谷氨酸到赖氨酸或甘氨酸的变化（大肠杆菌GyrA的对应位点）；这些变化在其他物种中与氟喹诺酮耐药性相关。在2株对环丙沙星耐药的屎肠球菌分离株（最低抑菌浓度为32微克/毫升）或4株实验室衍生的粪肠球菌自发环丙沙星耐药突变株（最低抑菌浓度为8至16微克/毫升）中，未发现所研究区域存在差异，这表明其他机制可能导致某些肠球菌对氟喹诺酮耐药。
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