el-Zaatari F A, Nguyen A M, Genta R M, Klein P D, Graham D Y
Department of Medicine, Veterans Affairs Medical Center, Houston, Texas 77030.
Dig Dis Sci. 1995 Jan;40(1):109-13. doi: 10.1007/BF02063952.
We previously reported the development and the possible application of reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of H. pylori in gastric mucosal biopsy specimens. In this communication, the application of this assay was assessed by comparing its results from 79 gastric biopsy specimens obtained from 68 patients with the more traditional [13C]urea breath test. When the amplified products were examined, the specificity and sensitivity of this RT-PCR assay were 100% and 47% on agarose gels and 80% and 91% by Southern hybridization, respectively. The specificity and sensitivity of urea breath test were 91% and 96% and were generally superior to RT-PCR (negative predictive value of 94% for UBT and 59-76% for RT-PCR). Although our RT-PCR results compare favorably with other PCR assays applied to gastric biopsy specimens for the detection of H. pylori, the use of this method did not add significantly to currently available noninvasive diagnostic methods.
我们之前报道了用于检测胃黏膜活检标本中幽门螺杆菌的逆转录-聚合酶链反应（RT-PCR）检测方法的开发及可能的应用。在本交流中，通过比较从68例患者获得的79份胃活检标本的该检测结果与更传统的[¹³C]尿素呼气试验结果，对该检测方法的应用进行了评估。当检查扩增产物时，该RT-PCR检测方法在琼脂糖凝胶上的特异性和敏感性分别为100%和47%，通过Southern杂交分别为80%和91%。尿素呼气试验的特异性和敏感性分别为91%和96%，总体上优于RT-PCR（尿素呼气试验的阴性预测值为94%，RT-PCR为59%-76%）。尽管我们的RT-PCR结果与应用于胃活检标本检测幽门螺杆菌的其他PCR检测方法相比具有优势，但该方法的使用并未显著增加目前可用的非侵入性诊断方法。
