微管中伴随GTP水解的结构变化：来自缓慢水解类似物鸟苷酰-（α，β）-亚甲基二膦酸酯的信息。
Structural changes accompanying GTP hydrolysis in microtubules: information from a slowly hydrolyzable analogue guanylyl-(alpha,beta)-methylene-diphosphonate.
作者信息
Hyman A A, Chrétien D, Arnal I, Wade R H
机构信息
Cell Biology Program, European Molecular Biology Laboratory, Heidelberg, Germany.
出版信息
J Cell Biol. 1995 Jan;128(1-2):117-25. doi: 10.1083/jcb.128.1.117.
Abstract
We have used cryoelectron microscopy to try to understand the structural basis for the role of GTP hydrolysis in destabilizing the microtubule lattice. We have measured a structural difference introduced into microtubules by replacing GTP with guanylyl-(alpha,beta)-methylene-diphosphonate (GMPCPP). In a stable GMPCPP microtubule lattice, the moiré patterns change and the tubulin subunits increase in size by 1.5 A. This information provides a clue to the role of hydrolysis in inducing the structural change at the end of a microtubule during the transition from a growing to a shrinking phase.
摘要
我们利用冷冻电子显微镜来试图理解GTP水解在破坏微管晶格稳定性中所起作用的结构基础。我们测量了用鸟苷酰 -（α，β）-亚甲基二磷酸酯（GMPCPP）取代GTP后引入微管的结构差异。在稳定的GMPCPP微管晶格中，莫尔条纹发生变化，微管蛋白亚基尺寸增大1.5埃。这一信息为水解在微管从生长阶段向收缩阶段转变过程中诱导微管末端结构变化所起的作用提供了线索。
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