Brandwein M, Zeitlin J, Nuovo G J, MacConnell P, Bodian C, Urken M, Biller H
Department of Pathology, University Hospital, State University of New York at Stony Brook.
Mod Pathol. 1994 Sep;7(7):720-7.
We examined the incidence of human papillomavirus (HPV) in intraoral cancers from 64 patients as determined by the highly sensitive technique of "hot start" polymerase chain reaction (PCR) in formalin-fixed paraffin-embedded tissues. Polymerase-chain-reaction-amplified HPV DNA was detected in the carcinomas of 16 patients (25%). The percentage of men in the HPV-positive (HPV+) group was greater than that in the HPV-negative (HPV-) group (86% versus 68%), but the difference was not statistically significant. There was no intraoral site preference for the HPV+ tumors. The mean age of viral-positive and -negative groups was similar (55 versus 53.8 yr). Three of 16 HPV+ patients (19%) had never smoked cigarettes; however, 16% of the HPV- group had also never smoked. Of interest, 38% of patients interviewed had occupation-related exposures that may have contributed to their carcinogenesis, and a disproportionate percentage of these patients (57%) were from the HPV+ group. There were no statistically significant differences between HPV+ and HPV- cases regarding T stage, clinical stage, and tumor differentiation. The disease-free interval did not differ significantly for HPV+ and HPV- patients in total nor when patients were stratified for tumor stage and clinical stage. The only group that showed some difference in outcome was that of the stage III/IV patients with oral cancer. We observed a shorter survival time for the HPV+ patients as compared with the HPV- patients (P = 0.09). We conclude that, in general, HPV is associated with a minority of intraoral cancers and its presence is not predictive of patient outcome.
我们采用“热启动”聚合酶链反应（PCR）这一高灵敏度技术，检测了64例口腔癌患者福尔马林固定石蜡包埋组织中的人乳头瘤病毒（HPV）感染率。在16例患者（25%）的癌组织中检测到聚合酶链反应扩增的HPV DNA。HPV阳性（HPV+）组男性所占百分比高于HPV阴性（HPV-）组（86%对68%），但差异无统计学意义。HPV+肿瘤在口腔内无部位偏好。病毒阳性组和阴性组的平均年龄相似（55岁对53.8岁）。16例HPV+患者中有3例（19%）从不吸烟；然而，HPV-组中也有16%的患者从不吸烟。有趣的是，接受访谈的患者中有38%有职业相关暴露，这可能促成了他们的癌症发生，而且这些患者中比例过高的（57%）来自HPV+组。HPV+和HPV-病例在T分期、临床分期和肿瘤分化方面无统计学显著差异。HPV+和HPV-患者的无病生存期总体上无显著差异，按肿瘤分期和临床分期分层时也无差异。唯一在预后方面显示出一些差异的是III/IV期口腔癌患者组。我们观察到，与HPV-患者相比，HPV+患者的生存时间较短（P = 0.09）。我们得出结论，总体而言，HPV与少数口腔癌相关，其存在并不能预测患者的预后。
