酵母中的UGA突变和UGA抑制基因

UGA mutations and UGA suppressors in yeast.

作者信息

Hawthorne D C

出版信息

Biochimie. 1976;58(1-2):179-82. doi: 10.1016/s0300-9084(76)80368-9.

Abstract

Eight UGA alleles have been obtained in S. cerevisiae. Two UGA alleles, ade5,7-143 and gal10-1, were isolated as forward mutations from wildtype. Six UGA alleles, arg4-2' (UGA), his5-2' (UGA), ilv3-1' (UGA), leu2'1' (UGA), lys1-1' (UGA), and asp5-1' (UGA), were derived from UAA alleles by selecting from revertants in the presence of UGA suppressors. With these 8 UGA alleles, two classes of UGA-specific suppressors can be distinguished. Suppressors in one class act upon all 8 allelqs ; while suppressors in the second class act upon all but 3 alleles : ade5,7-143, arg4-2' (UGA), and gal10-1.

摘要

在酿酒酵母中已获得8个UGA等位基因。两个UGA等位基因，ade5,7 - 143和gal10 - 1，是从野生型中分离得到的正向突变。六个UGA等位基因，arg4 - 2'（UGA）、his5 - 2'（UGA）、ilv3 - 1'（UGA）、leu2'1'（UGA）、lys1 - 1'（UGA）和asp5 - 1'（UGA），是通过在UGA抑制子存在的情况下从回复突变体中筛选，从UAA等位基因衍生而来。利用这8个UGA等位基因，可以区分出两类UGA特异性抑制子。一类抑制子作用于所有8个等位基因；而另一类抑制子作用于除ade5,7 - 143、arg4 - 2'（UGA）和gal10 - 1之外的所有等位基因。