Baie S H, Sheikh K A
Department of Pharmaceutical Technology, School of Pharmaceutical Sciences, Universiti Sains Malaysia, 11800, Penang, Malaysia.
J Ethnopharmacol. 2000 Jul;71(1-2):93-100. doi: 10.1016/s0378-8741(99)00184-1.
Channa striatus, a fresh water snakehead fish, is reported to enhance dermal wound healing. Biochemical components such as amino acids and fatty acids are important for the synthesis of collagen fibers during wound healing. Arachidonic acid, a precursor of prostaglandin plays a vital role in healing the wounds. Haruan (C. striatus) contains all the essential amino acids for wound healing particularly glycine as well as high contents of arachidonic acid and polyunsaturated fatty acids that can promote prostaglandin synthesis. In the present work we have studied the wound healing effect of C. striatus in Sprague-Dawley rats. Cream formulations having different haruan fish extract concentrations as the active ingredient were prepared and stabilized, and they were applied to the wounds. The healing of wounds was characterized by an increase in the tensile strength of the skin, determined on the 7th post-operative day in each case. Haruan treatment of wounds promotes remodeling of collagen, by the synthesis of inter- and intra-molecular protein crosslinking and thus produces a marked increase (P<0.05) in tensile strength as compared to the cetrimide treated group. On the basis of our experiment we conclude that C. striatus helps in wound healing as indicated by the increase in tensile strength. We hypothesise that this effect may be due to its high content of arachidonic acid, glycine and polyunsaturated fatty acids. The mechanism of wound healing will be investigated in future studies.
条纹鳢是一种淡水鳢鱼，据报道它能促进皮肤伤口愈合。氨基酸和脂肪酸等生化成分在伤口愈合过程中对胶原蛋白纤维的合成很重要。花生四烯酸作为前列腺素的前体，在伤口愈合中起着至关重要的作用。哈鲁安（条纹鳢）含有伤口愈合所需的所有必需氨基酸，尤其是甘氨酸，以及高含量的花生四烯酸和多不饱和脂肪酸，这些物质可以促进前列腺素的合成。在本研究中，我们研究了条纹鳢对斯普拉格-道利大鼠伤口愈合的影响。制备并稳定了以不同浓度的哈鲁安鱼提取物为活性成分的乳膏制剂，并将其应用于伤口。伤口愈合的特征是皮肤抗张强度增加，在每种情况下均于术后第7天测定。与西曲溴铵治疗组相比，用哈鲁安治疗伤口可通过分子间和分子内蛋白质交联的合成促进胶原蛋白重塑，从而使抗张强度显著增加（P<0.05）。根据我们的实验，我们得出结论，条纹鳢有助于伤口愈合，这表现为抗张强度的增加。我们推测这种作用可能归因于其高含量的花生四烯酸、甘氨酸和多不饱和脂肪酸。伤口愈合的机制将在未来的研究中进行探讨。
