抗坏血酸2-O-α-葡萄糖苷对发育中鸡胚氢化可的松诱导的白内障形成的影响：II. 对晶状体中谷胱甘肽和脂质过氧化物含量的影响

Effect of ascorbic acid 2-O-alpha-glucoside on hydrocortisone-induced cataract formation in developing chick embryos: II. Influence on glutathione and lipid peroxide contents in the lens.

作者信息

Nagata M, Hikida M, Mibu H, Muto N, Yamamoto I

机构信息

Central Research Laboratories, Santen Pharmaceutical Co., Ltd., Osaka, Japan.

出版信息

J Ocul Pharmacol. 1994 Fall;10(3):537-42. doi: 10.1089/jop.1994.10.537.

DOI:10.1089/jop.1994.10.537

PMID:7836862

Abstract

In developing chick embryos, hydrocortisone induces cataract formation following a decrease in lens glutathione content but an increase in lipid peroxide content in lens, blood and liver. The preventive effects of ascorbic acid 2-O-alpha-glucoside (AA-2G) on these parameters were compared on cataract formation with those of ascorbic acid (AsA) and ascorbic acid 2-O-phosphate (AA-2P). In these tissues, AA-2G inhibited a decrease in glutathione content and an increase in lipid peroxide content more effectively than either AsA or AA-2P. Various tissues including lens and liver have alpha-glucosidase activity, strongly suggesting that AsA is enzymatically liberated from AA-2G in these tissues. In summary, these results suggest that AA-2G exerts a potent anti-cataract activity via a reduction in oxidative damage through AsA release.

摘要

在发育中的鸡胚胎中，氢化可的松在晶状体谷胱甘肽含量降低但晶状体、血液和肝脏中脂质过氧化物含量增加后诱导白内障形成。将抗坏血酸2-O-α-葡糖苷（AA-2G）对这些参数的预防作用与抗坏血酸（AsA）和抗坏血酸2-O-磷酸酯（AA-2P）对白内障形成的预防作用进行了比较。在这些组织中，AA-2G比AsA或AA-2P更有效地抑制了谷胱甘肽含量的降低和脂质过氧化物含量的增加。包括晶状体和肝脏在内的各种组织都具有α-葡萄糖苷酶活性，这强烈表明AsA在这些组织中通过酶促作用从AA-2G中释放出来。总之，这些结果表明，AA-2G通过AsA释放减少氧化损伤，从而发挥强大的抗白内障活性。