Nishigori H, Lee J W, Yamauchi Y, Iwatsuru M
Curr Eye Res. 1986 Jan;5(1):37-40. doi: 10.3109/02713688608995163.
The level of lipid peroxide (LPO) in glucocorticoid-induced cataractous lenses was measured by using thiobarbituric acid. When 0.25 mumol of hydrocortisone hemisuccinate sodium (HC) were administered to 15-day-old chick embryos, the level of LPO in the lens increased to approximately 1.4-fold of the control level at 24 to 48 hr after HC treatment. However, the level returned to the control level by 96 hr with the disappearance of opacity in the lens. A triple application of ascorbic acid (20 mumol/egg) at 3, 10 and 20 hr after HC treatment prevented cataract formation and elevation in the level of LPO in 60% of the lenses. In 40% of the eggs treated with HC plus ascorbic acid, the results were no different than HC alone. Treatment with prednisolone hemisuccinate sodium (0.25 mumol/egg) produced an elevation in the level of LPO in the lens but that with cortisone hemisuccinate sodium or cortexolone hemisuccinate sodium did not change the level of LPO in the lens. The phenomena of the cataract formation and the elevation of LPO in the lens caused by HC seemed to be related to each other and due to its glucocorticoid activities.
采用硫代巴比妥酸法测定糖皮质激素诱导的白内障晶状体中脂质过氧化物（LPO）的水平。当向15日龄鸡胚注射0.25 μmol氢化可的松半琥珀酸钠（HC）时，晶状体中LPO水平在HC处理后24至48小时增加至对照水平的约1.4倍。然而，到96小时时该水平恢复到对照水平，晶状体中的浑浊消失。在HC处理后3、10和20小时三次施用抗坏血酸（20 μmol/蛋）可防止60%的晶状体形成白内障和LPO水平升高。在40%用HC加抗坏血酸处理的蛋中，结果与单独使用HC无异。用泼尼松龙半琥珀酸钠（0.25 μmol/蛋）处理可使晶状体中LPO水平升高，但用可的松半琥珀酸钠或皮质酮半琥珀酸钠处理则不会改变晶状体中LPO的水平。HC引起的晶状体白内障形成和LPO升高现象似乎相互关联，且归因于其糖皮质激素活性。
