Gomez G, Zhang T, Rajaraman S, Thakore K N, Yanaihara N, Townsend C M, Thompson J C, Greeley G H
Department of Surgery, University of Texas Medical Branch, Galveston 77555-0725.
Am J Physiol. 1995 Jan;268(1 Pt 1):G71-81. doi: 10.1152/ajpgi.1995.268.1.G71.
The purpose of this study was twofold: 1) to characterize the profile of colonic peptide YY (PYY) gene expression in rats and 2) to examine for potential trophic effects of PYY on the intestine in rats and mice. Expression of PYY mRNA (analyzed by Northern blotting and in situ hybridization) and PYY (analyzed by high-performance liquid chromatography and radioimmunoassay) was detected initially at day 17 of gestation in colonic extracts of Sprague-Dawley and Fischer rats. Expression of colonic PYY mRNA increased until 7 days of age and remained at its highest level (approximately twofold greater than the adult level) through the end of the nursing period. After weaning (21 days of age), PYY mRNA levels declined quickly to adult levels. Colonic PYY concentrations followed, in a coordinated manner, with some temporal delay after birth, the increase and decrease of its mRNA. Administration of PYY increased the weight and DNA content of the duodenum significantly in nursing rats and adult mice. In mice, PYY treatment also increased weight and DNA content of the ileum and colon. The trophic effects of PYY were dose related, peptide specific, and independent of species and sex. From these findings, we hypothesize that PYY plays an important role in intestinal development and dietary adaptation.
1）描述大鼠结肠肽YY（PYY）基因表达的特征；2）研究PYY对大鼠和小鼠肠道的潜在营养作用。最初在妊娠第17天，在斯普拉-道来大鼠和费希尔大鼠的结肠提取物中检测到PYY mRNA的表达（通过Northern印迹法和原位杂交分析）以及PYY（通过高效液相色谱法和放射免疫分析法分析）。结肠PYY mRNA的表达在出生后7日龄前持续增加，并在哺乳期结束时保持在最高水平（约为成年水平的两倍）。断奶后（21日龄），PYY mRNA水平迅速下降至成年水平。结肠PYY浓度以协调的方式在出生后有一定时间延迟地跟随其mRNA的增加和减少。给哺乳期大鼠和成年小鼠注射PYY可显著增加十二指肠的重量和DNA含量。在小鼠中，PYY处理还增加了回肠和结肠的重量和DNA含量。PYY的营养作用与剂量相关、具有肽特异性，且与物种和性别无关。基于这些发现，我们推测PYY在肠道发育和饮食适应中起重要作用。
