Zhang Wei, Li Ning, Zhu Weiming, Shi Yichao, Zhang Jian, Li Qiurong, Li Jieshou
Research Institute of General Surgery, Jinling Hospital, Medical School of Nanjing University, Nanjing, China.
Pediatr Surg Int. 2008 Aug;24(8):913-9. doi: 10.1007/s00383-008-2176-y. Epub 2008 May 30.
The main reason why enterocyte proliferative effects of peptide YY (PYY) have not been detected in rats undergoing massive small intestinal resection after feeding may have been the background activity of markedly increased endogenous PYY released from L cells in the distal gut in response to the intraluminal nutrients. The purpose of the present study was to evaluate the effects of PYY on enterocyte proliferation in a rat model of distal bowel resection (DBR) with total enteral nutrition (TEN). Male, adult Sprague-Dawley rats were assigned into three experimental groups: sham rats underwent bowel transection and reanastomosis, DBR rats underwent the resection of 40 cm distal small intestine and colon, and DBR-PYY rats underwent distal bowel resection as above, and were treated with PYY(1-36) from day 2 to day 14 postoperatively. During days 2-14 postoperatively, all animals received isocaloric TEN. At the endpoints, plasma PYY levels and parameters of enterocyte proliferation were determined. Compared with the sham group, DBR rats demonstrated a significant decrease in plasma PYY levels, and a significant increase in intestinal bowel and mucosal weight, mucosal DNA and protein content, villus height and crypt depth, and crypt cell proliferation index. Administration of PYY (DBR-PYY group) led to a significant increase in plasma PYY levels, intestinal bowel and mucosal weight, mucosal DNA and protein content, villus height and crypt depth, and crypt cell proliferation index in comparison with the DBR untreated group. We conclude that administration of PYY increases the plasma PYY levels, and PYY induces enterocyte proliferation with TEN after distal bowel resection.
喂食后在接受大量小肠切除的大鼠中未检测到肽YY（PYY）对肠上皮细胞增殖作用的主要原因，可能是远端肠道的L细胞响应肠腔内营养物质释放出的内源性PYY显著增加所产生的背景活性。本研究的目的是评估PYY对全肠内营养（TEN）的远端肠切除（DBR）大鼠模型中肠上皮细胞增殖的影响。将成年雄性Sprague-Dawley大鼠分为三个实验组：假手术组大鼠进行肠横断和再吻合术；DBR组大鼠切除远端40 cm小肠和结肠；DBR-PYY组大鼠进行上述远端肠切除，并在术后第2天至第14天用PYY（1-36）治疗。术后第2天至第14天，所有动物均接受等热量的TEN。在实验终点，测定血浆PYY水平和肠上皮细胞增殖参数。与假手术组相比，DBR组大鼠血浆PYY水平显著降低，肠管和黏膜重量、黏膜DNA和蛋白质含量、绒毛高度和隐窝深度以及隐窝细胞增殖指数显著增加。与未治疗的DBR组相比，给予PYY（DBR-PYY组）导致血浆PYY水平、肠管和黏膜重量、黏膜DNA和蛋白质含量、绒毛高度和隐窝深度以及隐窝细胞增殖指数显著增加。我们得出结论，给予PYY可提高血浆PYY水平，并且PYY在远端肠切除后通过TEN诱导肠上皮细胞增殖。
