Gianneli D, Tzelepi E, Tzouvelekis L S, Mentis A F, Nikolopoulou C
Department of Microbiology, Tzannion General Hospital, Piraeus, Greece.
Eur J Clin Microbiol Infect Dis. 1994 Sep;13(9):764-7. doi: 10.1007/BF02276063.
The resistance to third generation cephalosporins in nine Serratia marcescens strains isolated in Greek hospitals was studied. Eight of the strains transferred resistance to Escherichia coli by means of large plasmids that encoded for an extended-spectrum beta-lactamase. Hybridization, isoelectric focusing and hydrolysis studies showed that the enzyme resembled the SHV-5 beta-lactamase. In the eight isolates that possessed the SHV type enzyme, cephalosporinase expression was inducible, whereas the remaining strain was a cephalosporinase hyperproducing strain. Introduction of a plasmid coding for the regulatory ampD gene in the latter strain eliminated beta-lactamase production and rendered the strain susceptible to cephalosporins.
对从希腊医院分离出的9株粘质沙雷氏菌菌株对第三代头孢菌素的耐药性进行了研究。其中8株菌株通过编码超广谱β-内酰胺酶的大质粒将耐药性转移至大肠杆菌。杂交、等电聚焦和水解研究表明，该酶与SHV-5β-内酰胺酶相似。在拥有SHV型酶的8株分离株中，头孢菌素酶的表达是可诱导的，而其余菌株是头孢菌素酶高产菌株。在后一种菌株中引入编码调节性ampD基因的质粒消除了β-内酰胺酶的产生，并使该菌株对头孢菌素敏感。
