Ostrow R S, Coughlin S M, McGlennen R C, Johnson A N, Ratterree M S, Scheffler J, Yaegashi N, Galloway D A, Faras A J
Institute of Human Genetics, University of Minnesota Medical School, Minneapolis 55455.
J Gen Virol. 1995 Feb;76 ( Pt 2):293-9. doi: 10.1099/0022-1317-76-2-293.
We have previously demonstrated the presence of rhesus monkey papillomavirus type 1 (RhPV-1), from molecular and pathological evidence, in a mating group within a single institution. We have now also obtained a number of fresh or archival tissues of rhesus monkeys from other geographically distinct institutions. Using PCR amplification, we observed two animals from one of these institutions and five animals from another which demonstrated RhPV-1 DNA sequences. In addition we molecularly cloned the E7, E2, E4, L2 and L1 genes of RhPV-1 into bacterial expression vectors. The fusion gene products were used to test for serological response to RhPV-1 antigens by Western blot analysis. Responses were observed in up to 52% of the animals tested. While some serologically positive animals were also RhPV-1 DNA-positive, most were not.
我们之前已通过分子和病理学证据证明，在单一机构内的一个交配群体中存在恒河猴乳头瘤病毒1型（RhPV-1）。我们现在还从其他地理位置不同的机构获得了一些恒河猴的新鲜或存档组织。通过PCR扩增，我们在其中一个机构的两只动物以及另一个机构的五只动物中观察到了RhPV-1 DNA序列。此外，我们将RhPV-1的E7、E2、E4、L2和L1基因分子克隆到细菌表达载体中。融合基因产物用于通过蛋白质印迹分析检测对RhPV-1抗原的血清学反应。在高达52%的受试动物中观察到了反应。虽然一些血清学阳性的动物也是RhPV-1 DNA阳性，但大多数并非如此。
