Schneider J F, McGlennen R C, LaBresh K V, Ostrow R S, Faras A J
Department of Microbiology, University of Minnesota Medical School, Minneapolis 55455.
J Virol. 1991 Jun;65(6):3354-8. doi: 10.1128/JVI.65.6.3354-3358.1991.
Rhesus Papillomavirus type 1 (RhPV-1) was recently cloned from a rhesus monkey lymph node metastasis of a penile squamous cell carcinoma. In this paper, we demonstrate that RhPV-1 cooperates with the activated ras oncogene to transform primary cells at a level comparable to human papillomavirus type 16. The viral DNAs were cloned such that their expression was under the control of their natural promoter elements. Unlike human papillomavirus type 16, RhPV-1 DNA cooperated with ras independently of the hormone dexamethasone. However, dexamethasone did have a positive influence on the ability of some RhPV-1 cotransformed cells to grow in soft-agar assays. The transformed cells are highly tumorigenic in vivo in nude mice.
恒河猴乳头瘤病毒1型（RhPV-1）最近从一只患有阴茎鳞状细胞癌的恒河猴淋巴结转移灶中克隆出来。在本文中，我们证明RhPV-1与激活的ras癌基因协同作用，以与16型人乳头瘤病毒相当的水平转化原代细胞。病毒DNA被克隆，使其表达受其天然启动子元件的控制。与16型人乳头瘤病毒不同，RhPV-1 DNA与ras协同作用，不依赖于激素地塞米松。然而，地塞米松确实对一些RhPV-1共转化细胞在软琼脂试验中的生长能力有积极影响。转化后的细胞在裸鼠体内具有高度致瘤性。
