Shimazaki J, Tsubota K, Sawa M, Kinoshita S, Ohkura T, Honda M
Department of Ophthalmology, Tokyo Dental College, Chiba, Japan.
Br J Ophthalmol. 1994 Nov;78(11):859-62. doi: 10.1136/bjo.78.11.859.
Detection of human immunodeficiency virus (HIV), hepatitis B virus (HBV), and hepatitis C virus (HCV) in donor eyes was performed. DNAs were extracted from the uvea, and they were amplified using the polymerase chain reaction (PCR). Amplified viral DNAs were detected with liquid hybridisation and chemiluminescent assay in which no radioactive materials were used. This method was shown to have a sensitivity limit of fewer than 10 copies of HIV, making it much more sensitive than the current techniques employed in eye banks. The method was applied to 120 donor eyes, including four from donors seropositive for HBV. The HBV gene was detected in one case in which the donor's blood had not been tested for HBV. HIV and HCV genes were not detected in any of the samples. The assay could be an effective screening test for the detection of these viruses in eye bank eyes.
对供体眼进行了人类免疫缺陷病毒（HIV）、乙型肝炎病毒（HBV）和丙型肝炎病毒（HCV）的检测。从葡萄膜中提取DNA，并使用聚合酶链反应（PCR）进行扩增。使用液体杂交和化学发光测定法检测扩增的病毒DNA，该方法不使用放射性物质。结果表明，该方法的灵敏度极限低于10份HIV拷贝，比眼库目前采用的技术灵敏得多。该方法应用于120只供体眼，其中包括4只来自HBV血清学阳性供体的眼睛。在1例供体血液未检测HBV的病例中检测到了HBV基因。在任何样本中均未检测到HIV和HCV基因。该检测方法可能是眼库供体眼中检测这些病毒的有效筛查试验。
