• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

噬菌体φX174的感染过程XLI. 15摄氏度下缺陷型φX颗粒的合成

Process of infection with bacteriophage phi X174 XLI. Synthesis of defective phi X particles at 15 degrees C.

作者信息

Espejo R T, Sinsheimer R L

出版信息

J Virol. 1976 Aug;19(2):732-42. doi: 10.1128/JVI.19.2.732-742.1976.

DOI:10.1128/JVI.19.2.732-742.1976
PMID:785025
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC354906/
Abstract

At 15 degrees C, phi X174-infected cells make single-stranded viral DNA fragments, varying in size from 0.2 to 0.9 times that of phi X DNA. In non-deproteinized lysates, this single-stranded DNA is found associated with proteins in particles sedimenting heterogeneously with an S20, w average of 80 to 90S. These particles do not differ appreciably from mature virus in polypeptide composition. Chase experiments, at 37 degrees C, of the label incorporated into this DNA at 15 degrees C suggest that both the single-stranded DNA fragments and the 80 to 90S particles are not precursors of virions but are defective end products.

摘要

在15摄氏度时，被φX174感染的细胞产生单链病毒DNA片段，其大小在φX DNA的0.2至0.9倍之间变化。在未脱蛋白的裂解物中，这种单链DNA与蛋白质结合，存在于沉降不均一的颗粒中，其S20,w平均值为80至90S。这些颗粒在多肽组成上与成熟病毒没有明显差异。对在15摄氏度时掺入该DNA的标记物进行37摄氏度的追踪实验表明，单链DNA片段和80至90S颗粒都不是病毒体的前体，而是有缺陷的终产物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a15d/354906/90089fb3795f/jvirol00224-0460-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a15d/354906/df50147fe13c/jvirol00224-0456-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a15d/354906/90089fb3795f/jvirol00224-0460-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a15d/354906/df50147fe13c/jvirol00224-0456-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a15d/354906/90089fb3795f/jvirol00224-0460-a.jpg

相似文献

1
Process of infection with bacteriophage phi X174 XLI. Synthesis of defective phi X particles at 15 degrees C.噬菌体φX174的感染过程XLI. 15摄氏度下缺陷型φX颗粒的合成
J Virol. 1976 Aug;19(2):732-42. doi: 10.1128/JVI.19.2.732-742.1976.
2
DNA synthesis in Escherichia coli cells infected with gene H mutants of bacteriophage phi X174.感染噬菌体phi X174基因H突变体的大肠杆菌细胞中的DNA合成。
J Virol. 1979 Mar;29(3):973-82. doi: 10.1128/JVI.29.3.973-982.1979.
3
The rep mutation. II. Its effect on Escherichia coli and on the replication of bacteriophage phi X174.rep突变。II. 其对大肠杆菌及噬菌体φX174复制的影响。
Virology. 1972 Aug;49(2):486-96. doi: 10.1016/0042-6822(72)90500-4.
4
Assembly of bacteriophage phi X174: identification of a virion capsid precursor and proposal of a model for the functions of bacteriophage gene products during morphogenesis.噬菌体φX174的组装：病毒粒子衣壳前体的鉴定及噬菌体基因产物在形态发生过程中功能模型的提出。
J Virol. 1977 Oct;24(1):303-13. doi: 10.1128/JVI.24.1.303-313.1977.
5
Viral DNA-synthesizing intermediate complex isolated during assembly of bacteriophage phi X174.在噬菌体φX174组装过程中分离出的病毒DNA合成中间复合物。
J Virol. 1976 Aug;19(2):409-15. doi: 10.1128/JVI.19.2.409-415.1976.
6
Mechanism of replication of phi x174 single-stranded DNA. IX. Requirement for the Escherichia coli dnaG protein.φX174单链DNA的复制机制。IX. 对大肠杆菌dnaG蛋白的需求
J Virol. 1974 Nov;14(5):1070-5. doi: 10.1128/JVI.14.5.1070-1075.1974.
7
A role for single-strand breaks in bacteriophage phi-X174 genetic recombination.单链断裂在噬菌体φ-X174基因重组中的作用。
J Mol Biol. 1974 Sep 25;88(3):629-51. doi: 10.1016/0022-2836(74)90414-8.
8
Identification of the block in the intracellular replication of single-stranded DNA of photodynamically inactivated bacteriophage phi X174.光动力学灭活噬菌体φX174单链DNA细胞内复制阻滞的鉴定
J Virol. 1973 Mar;11(3):368-71. doi: 10.1128/JVI.11.3.368-371.1973.
9
phi X174-directed DNA and protein syntheses in infected minicells.φX174噬菌体感染微小细胞后所引发的DNA与蛋白质合成
J Virol. 1981 Nov;40(2):396-402. doi: 10.1128/JVI.40.2.396-402.1981.
10
In vitro system that synthesizes circular viral DNA of bacteriophage phi X 174.合成噬菌体φX 174环状病毒DNA的体外系统。
J Virol. 1977 Jun;22(3):619-25. doi: 10.1128/JVI.22.3.619-625.1977.

本文引用的文献

1
Direction of Translation and Size of Bacteriophage phiX174 Cistrons.噬菌体φX174顺反子的翻译方向和大小
J Virol. 1972 Jul;10(1):99-114. doi: 10.1128/JVI.10.1.99-114.1972.
2
The process of infection with bacteriophage phi-X174. X. Mutations in a phi-X Lysis gene.噬菌体φ-X174的感染过程。X. φ-X裂解基因中的突变
J Mol Biol. 1966 Jul;18(3):429-47. doi: 10.1016/s0022-2836(66)80035-9.
3
The process of infection with bacteriophage phi-X174 VII. Ultracentrifugal analysis of the replicative form.噬菌体φ-X174的感染过程 VII. 复制型的超速离心分析
J Mol Biol. 1965 Dec;14(2):327-47. doi: 10.1016/s0022-2836(65)80185-1.
4
Phi-X-174 bacteriophage structural mutants which affect deoxyribonucleic acid synthesis.影响脱氧核糖核酸合成的Φ-X-174噬菌体结构突变体。
J Virol. 1969 Oct;4(4):400-7. doi: 10.1128/JVI.4.4.400-407.1969.
5
Mechanism of replication of phi-X-174 single stranded DNA.噬菌体φ-X-174单链DNA的复制机制
Nature. 1968 Jul 27;219(5152):346-51. doi: 10.1038/219346a0.
6
The process of infection with Bacteriophage phi-X174. XXIX. In vivo studies on the synthesis of the single-stranded DNA of progeny phi-X174 bacteriophage.噬菌体φ-X174的感染过程。二十九。子代φ-X174噬菌体单链DNA合成的体内研究。
J Mol Biol. 1969 Oct 28;45(2):237-63. doi: 10.1016/0022-2836(69)90103-x.
7
The rolling circle for phiX DNA replication. II. Synthesis of single-stranded circles.噬菌体φX DNA复制的滚环机制。II. 单链环的合成
Proc Natl Acad Sci U S A. 1970 Dec;67(4):1934-42. doi: 10.1073/pnas.67.4.1934.
8
Gel electrophoretic separation of the complementary strands of bacteriophage DNA.噬菌体DNA互补链的凝胶电泳分离
Virology. 1972 Jul;49(1):342-4. doi: 10.1016/s0042-6822(72)80042-4.
9
Fate of parental phi X174-DNA upon infection of starved thymine-requiring host cells.饥饿的胸腺嘧啶需求型宿主细胞被感染后亲本φX174-DNA的命运
Virology. 1971 Apr;44(1):168-87. doi: 10.1016/0042-6822(71)90163-2.
10
A quantitative assay for DNA-RNA hybrids with DNA immobilized on a membrane.一种用于检测固定在膜上的DNA与RNA杂交体的定量分析方法。
J Mol Biol. 1965 Jul;12(3):829-42. doi: 10.1016/s0022-2836(65)80331-x.