Boris-Lawrie K, Temin H M
McArdle Laboratory for Cancer Research, University of Wisconsin Medical School, Madison.
J Virol. 1995 Mar;69(3):1920-4. doi: 10.1128/JVI.69.3.1920-1924.1995.
Retrovirus genomes have a conserved modular organization that consists of trans-acting gag, pol, and env genes that function through cis-acting sequences to replicate the RNA genome to the DNA provirus. Genetically more complex retroviruses also encode regulatory genes and cis-acting sequences that are essential for their replication. We sought to convert a more complex retrovirus into a simpler retrovirus derivative that can replicate. We constructed novel, hybrid retrovirus vectors to replicate the gag, pol, and env genes of the more complex bovine leukemia virus (BLV) in the absence of regulatory genes and cis-acting response sequences. Most of the cis-acting sequences involved in the replication and regulation of BLV were replaced by the cis-acting transcriptional control sequences of a simpler retrovirus, spleen necrosis virus. We found that the resulting hybrid BLV derivatives can replicate independently of BLV Tax and Rex and the Tax and Rex cis-acting response sequences, as measured by successive passages of virus on target cells, detection of provirus sequences, and analyses of provirus and encapsidated RNAs.
逆转录病毒基因组具有保守的模块化结构，由反式作用的gag、pol和env基因组成，这些基因通过顺式作用序列发挥功能，将RNA基因组复制为DNA前病毒。遗传上更复杂的逆转录病毒还编码对其复制至关重要的调控基因和顺式作用序列。我们试图将一种更复杂的逆转录病毒转化为一种能够复制的更简单的逆转录病毒衍生物。我们构建了新型杂交逆转录病毒载体，以在没有调控基因和顺式作用反应序列的情况下复制更复杂的牛白血病病毒（BLV）的gag、pol和env基因。参与BLV复制和调控的大多数顺式作用序列被一种更简单的逆转录病毒——脾坏死病毒的顺式作用转录控制序列所取代。我们发现，通过在靶细胞上连续传代病毒、检测前病毒序列以及分析前病毒和衣壳化RNA，所产生的杂交BLV衍生物能够独立于BLV Tax和Rex以及Tax和Rex顺式作用反应序列进行复制。
