v-Rel转化的淋巴细胞胞质溶胶中组成型和诱导型κB结合活性
Constitutive and inducible kappa B binding activities in the cytosol of v-Rel-transformed lymphoid cells.
作者信息
Hodgson J, Enrietto P J
机构信息
Department of Microbiology, State University of New York, Stony Brook 11794.
出版信息
J Virol. 1995 Mar;69(3):1971-9. doi: 10.1128/JVI.69.3.1971-1979.1995.
Abstract
Constitutive and inducible kapp B binding activities associated with v-Rel and c-Rel in the cytosol of v-Rel-transformed cells have been identified. These activities were resolved by electrophoretic mobility shift assay and chromatographic techniques into a high-molecular-weight protein-DNA complex designated complex I containing v- and c-Rel and lower-molecular-weight complexes II, III and IV which contained only v-Rel and which were stimulated by nucleotides, low pH, and detergent. These experiments suggest that interaction of v-Rel and c-Rel decreases the DNA-binding activity of each.
摘要
已鉴定出在v-Rel转化细胞的细胞质中与v-Rel和c-Rel相关的组成型和诱导型κB结合活性。通过电泳迁移率变动分析和色谱技术，这些活性被解析为一种高分子量的蛋白质-DNA复合物，命名为复合物I，其包含v-Rel和c-Rel，以及低分子量的复合物II、III和IV，这些复合物仅包含v-Rel，并且受到核苷酸、低pH值和去污剂的刺激。这些实验表明，v-Rel和c-Rel的相互作用降低了各自的DNA结合活性。
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