采用气相色谱/质谱联用仪（GC/MS）和放射免疫分析（RIA）法检测尿液、血清及毛发中的6-单乙酰吗啡。

The detection of 6-monoacetylmorphine in urine, serum and hair by GC/MS and RIA.

作者信息

Moeller M R, Mueller C

机构信息

Institute of Legal Medicine, Homburg, Germany.

出版信息

Forensic Sci Int. 1995 Jan 5;70(1-3):125-33. doi: 10.1016/0379-0738(94)01613-a.

DOI:10.1016/0379-0738(94)01613-a

PMID:7860024

Abstract

6-Monoacetylmorphine (6-MAM) is a good indicator for the intake of heroin and can be detected in blood, urine and hair of heroin users. A new radioimmunoassay (RIA) designed specifically for 6-monoacetylmorphine (6-MAM) was tested for its usefulness for the quantitation of the drug in urine, serum and hair. Its cross-reactivity with heroin and its metabolites, and related compounds was also determined. Eighty-nine hair, six serum and 25 urine samples where 6-MAM had been previously identified by GC/MS were analysed for 6-MAM with the new RIA kit. A good correlation existed between the GC/MS and RIA results for the hair samples. However, the amount of 6-MAM found in serum and urine differed considerably between the two methods. This difference could be explained by the cross-reactivity of the antibody with morphine and morphine-6-glucuronide, which are present in much larger amounts in serum and urine, than in hair. To evaluate a new rationalisation procedure, some hair samples were split into two portions after incubation. One part was analyzed for 6-MAM by RIA, and the other portion by GC/MS.

摘要

6-单乙酰吗啡（6-MAM）是海洛因摄入的良好指标，可在海洛因使用者的血液、尿液和毛发中检测到。一种专门为6-单乙酰吗啡（6-MAM）设计的新型放射免疫分析（RIA）方法，对其在尿液、血清和毛发中定量检测该药物的实用性进行了测试。还测定了其与海洛因及其代谢物以及相关化合物的交叉反应性。使用新型RIA试剂盒，对89份毛发、6份血清和25份尿液样本进行了分析，这些样本之前已通过气相色谱/质谱联用仪（GC/MS）鉴定出含有6-MAM。毛发样本的GC/MS结果与RIA结果之间存在良好的相关性。然而，两种方法在血清和尿液中检测到的6-MAM量差异很大。这种差异可以用抗体与吗啡和吗啡-6-葡萄糖醛酸苷的交叉反应来解释，它们在血清和尿液中的含量比在毛发中要高得多。为了评估一种新的合理化程序，一些毛发样本在孵育后被分成两部分。一部分通过RIA分析6-MAM，另一部分通过GC/MS分析。