抗狂犬病人类单克隆抗体的研发。

Development of human monoclonal antibodies to rabies.

作者信息

Dorfman N, Dietzschold B, Kajiyama W, Fu Z F, Koprowski H, Notkins A L

机构信息

Laboratory of Oral Medicine, National Institute of Dental Research, National Institutes of Health, Bethesda, Maryland 20892.

出版信息

Hybridoma. 1994 Oct;13(5):397-402. doi: 10.1089/hyb.1994.13.397.

DOI:10.1089/hyb.1994.13.397

PMID:7860096

Abstract

A total of nine human monoclonal antibodies (MAbs) to rabies virus were generated from peripheral B lymphocytes of subjects immunized with human diploid cell rabies vaccine by somatic cell hybridization. The MAbs were analyzed for their antigen-binding specificities using ELISA, Western blot, and immunoprecipitation assays. The different assays made it possible to identify MAbs directed to the surface glycoprotein, nucleoprotein, nominal phosphoprotein, and matrix protein. One of the MAbs that recognized the surface glycoprotein neutralized rabies virus.

摘要

通过体细胞杂交技术，从接种人二倍体细胞狂犬病疫苗的受试者外周血B淋巴细胞中总共产生了9种抗狂犬病病毒的人单克隆抗体（MAb）。使用酶联免疫吸附测定（ELISA）、蛋白质免疫印迹法（Western blot）和免疫沉淀试验分析这些单克隆抗体的抗原结合特异性。不同的试验使得鉴定针对表面糖蛋白、核蛋白、标称磷蛋白和基质蛋白的单克隆抗体成为可能。其中一种识别表面糖蛋白的单克隆抗体可中和狂犬病病毒。