大肠杆菌氮调节蛋白I-磷酸的去磷酸化激活
Activation of the dephosphorylation of nitrogen regulator I-phosphate of Escherichia coli.
作者信息
Liu J, Magasanik B
机构信息
Department of Biology, Massachusetts Institute of Technology, Cambridge, 02139.
出版信息
J Bacteriol. 1995 Feb;177(4):926-31. doi: 10.1128/jb.177.4.926-931.1995.
Abstract
The transcription of sigma 54 RNA polymerase-dependent nitrogen-regulated genes is activated by nitrogen regulator I (NRI)-phosphate. The kinase NRII is responsible for the phosphorylation of NRI. It has been shown that NRII also has the ability to dephosphorylate NRI-phosphate but only when PII is present at a concentration greatly in excess of that of NRII. We have now shown that glutamate enables PII to stimulate the dephosphorylation of NRI-phosphate when present in equimolar concentration with NRII. This effect of glutamate appears to be a backup control that becomes effective when the normal regulation of PII activity is disabled.
摘要
σ54 RNA聚合酶依赖性氮调节基因的转录由氮调节因子I（NRI）-磷酸盐激活。激酶NRII负责NRI的磷酸化。已经表明，NRII也有使NRI-磷酸盐去磷酸化的能力，但仅当PII的浓度大大超过NRII时才会如此。我们现在已经表明，当谷氨酸与NRII以等摩尔浓度存在时，它能使PII刺激NRI-磷酸盐的去磷酸化。谷氨酸的这种作用似乎是一种备用控制，当PII活性的正常调节被破坏时变得有效。
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本文引用的文献
1
The glnB region of the Escherichia coli chromosome.大肠杆菌染色体的glnB区域。
J Bacteriol. 1993 Nov;175(22):7441-9. doi: 10.1128/jb.175.22.7441-7449.1993.
2
Interdependence of K+ and glutamate accumulation during osmotic adaptation of Escherichia coli.大肠杆菌渗透适应过程中钾离子与谷氨酸积累的相互依存关系。
J Biol Chem. 1994 Jan 21;269(3):1911-7.
3
Regulation of the synthesis of glutamine synthetase by the PII protein in Klebsiella aerogenes.产气克雷伯菌中PII蛋白对谷氨酰胺合成酶合成的调控
Proc Natl Acad Sci U S A. 1980 May;77(5):2636-40. doi: 10.1073/pnas.77.5.2636.
4
Purification of nitrogen regulator II, the product of the glnL (ntrB) gene of Escherichia coli.大肠杆菌谷氨酰胺合成酶基因（ntrB）产物氮调节蛋白II的纯化
J Bacteriol. 1986 Nov;168(2):1002-4. doi: 10.1128/jb.168.2.1002-1004.1986.
5
New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues.对含有相邻组氨酸残基的蛋白质和肽具有选择性的新型金属螯合吸附剂。
J Chromatogr. 1987 Dec 18;411:177-84. doi: 10.1016/s0021-9673(00)93969-4.
6
7
Covalent modification of the glnG product, NRI, by the glnL product, NRII, regulates the transcription of the glnALG operon in Escherichia coli.谷氨酰胺合成酶L（glnL）的产物NRII对谷氨酰胺合成酶G（glnG）的产物NRI进行共价修饰，从而调控大肠杆菌中谷氨酰胺合成酶A、B、C操纵子（glnALG operon）的转录。
Proc Natl Acad Sci U S A. 1986 Aug;83(16):5909-13. doi: 10.1073/pnas.83.16.5909.
8
Transcription of glnA by purified Escherichia coli components: core RNA polymerase and the products of glnF, glnG, and glnL.用纯化的大肠杆菌成分转录谷氨酰胺合成酶基因A：核心RNA聚合酶以及谷氨酰胺合成酶基因F、谷氨酰胺合成酶基因G和谷氨酰胺合成酶基因L的产物。
Proc Natl Acad Sci U S A. 1985 Dec;82(24):8453-7. doi: 10.1073/pnas.82.24.8453.
9
Role of glnB and glnD gene products in regulation of the glnALG operon of Escherichia coli.谷氨酰胺B和谷氨酰胺D基因产物在大肠杆菌谷氨酰胺合成酶操纵子调节中的作用。
J Bacteriol. 1985 Nov;164(2):816-22. doi: 10.1128/jb.164.2.816-822.1985.
10
Phosphorylation of nitrogen regulator I (NRI) of Escherichia coli.
Proc Natl Acad Sci U S A. 1988 Dec;85(23):8919-23. doi: 10.1073/pnas.85.23.8919.
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