Carlin A, Shi W, Dey S, Rosen B P
Department of Biochemistry, Wayne State University School of Medicine, Detroit, Michigan 48201.
J Bacteriol. 1995 Feb;177(4):981-6. doi: 10.1128/jb.177.4.981-986.1995.
The chromosomally encoded arsenical resistance (ars) operon subcloned into a multicopy plasmid was found to confer a moderate level of resistance to arsenite and antimonite in Escherichia coli. When the operon was deleted from the chromosome, the cells exhibited hypersensitivity to arsenite, antimonite, and arsenate. Expression of the ars genes was inducible by arsenite. By Southern hybridization, the operon was found in all strains of E. coli examined but not in Salmonella typhimurium, Pseudomonas aeruginosa, or Bacillus subtilis.
将染色体编码的抗砷（ars）操纵子亚克隆到多拷贝质粒中，发现其可使大肠杆菌对亚砷酸盐和亚锑酸盐产生中等水平的抗性。当从染色体上删除该操纵子时，细胞对亚砷酸盐、亚锑酸盐和砷酸盐表现出超敏性。ars基因的表达可被亚砷酸盐诱导。通过Southern杂交发现，在所检测的所有大肠杆菌菌株中都存在该操纵子，但在鼠伤寒沙门氏菌、铜绿假单胞菌或枯草芽孢杆菌中不存在。
