Murakami Y, Kato A, Tsuda T, Morozumi T, Miura Y, Sugimura T
Exotic Disease Research Division, National Institute of Animal Health, Tokyo, Japan.
J Vet Med Sci. 1994 Oct;56(5):891-4. doi: 10.1292/jvms.56.891.
Three porcine reproductive and respiratory syndrome viruses (PRRSVs) were successfully isolated from stillborn piglets and fattening pigs derived from different herds affected either with an epizootic reproductive failure or a severe chronic respiratory distress in Japan. The isolates were reacted with antisera against both European (Lelystad virus) and American (strain 46448) PRRSVs in indirect immunofluorescence (IIF). However, cross immunoperoxidase monolayer assay revealed the close serological relationships between the Japanese isolates and American PRRSV, but not between the Japanese isolates and the European PRRSV. The data of preliminary serological survey with IIF technique showed a high frequency of antibody positive pigs against both Japanese isolate and the American PRRSV, whereas a low frequency of antibody positive pigs against the European PRRSV, on herds which have had clinical episodes of PRRS-like diseases. These results indicate that PRRSVs are prevalent in Japan, and suggest that the antigenicities of the prevalent PRRSVs are more closely related to those of the American than the European PRRSVs.
从日本不同猪群的死胎仔猪和育肥猪中成功分离出三株猪繁殖与呼吸综合征病毒（PRRSV），这些猪群分别受到流行性繁殖障碍或严重慢性呼吸窘迫的影响。将分离株与抗欧洲型（莱利斯塔德病毒）和美洲型（46448株）PRRSV的抗血清进行间接免疫荧光试验（IIF）。然而，交叉免疫过氧化物酶单层试验显示日本分离株与美洲型PRRSV之间存在密切的血清学关系，而与欧洲型PRRSV之间不存在这种关系。用IIF技术进行的初步血清学调查数据显示，在曾有类似PRRS疾病临床发作的猪群中，针对日本分离株和美洲型PRRSV的抗体阳性猪频率较高，而针对欧洲型PRRSV的抗体阳性猪频率较低。这些结果表明PRRSV在日本流行，并且提示流行的PRRSV的抗原性与美洲型PRRSV的抗原性比与欧洲型PRRSV的抗原性更密切相关。
