Popov T A, Velikiĭ N N, Parkhomets P K
Ukr Biokhim Zh. 1976 Mar-Apr;48(2):200-5.
Perfusion of the rat liver in situ for 150 min provides for maintaining optimal values of acid-base balance for the following indexes: surplus of bases, content of standard bicarbonate, buffer bases pH, pO2, pCO2, HbO2, the level of bile secretion, content of lactate, pyruvate, ATP, ADP, that evidences for a high functional activity in the tissue. Introduction of dinitro-ortho-cresol (DNC) into the perfusion liquid causes development of acidosis. DNC results in dissociation of oxidative phosphorylation: the content of ATP and intensity of inorganic phosphorus utilization decrease, oxygen uptake intensifies. A compensatory increase in the glycolysis intensity directed to maintaining the level of macroergs under these conditions is is pronounced in the intensified uptake of glucose, in a rise in the content of lactate in perfusate and an increase in the pyruvate kinase activity in the liver. The redox state of NAD-pairs (ratio of [NAD+] : [NADN] calculated from the content of redox metabolites and the equilibrium constant for the lactate dehydrogenase system shifts toward an increase in the reducing properties of hepatocytes cytoplasm. The phosphate potential value calculated from the ratio [ATP] : [ADP] - [Pinor] lowers under conditions of the experiment.
大鼠肝脏原位灌注150分钟可维持以下酸碱平衡指标的最佳值：碱剩余、标准碳酸氢盐含量、缓冲碱、pH值、pO₂、pCO₂、HbO₂、胆汁分泌水平、乳酸、丙酮酸、ATP、ADP含量，这表明组织具有较高的功能活性。向灌注液中加入二硝基邻甲酚（DNC）会导致酸中毒。DNC导致氧化磷酸化解离：ATP含量和无机磷利用强度降低，氧摄取增强。在这些条件下，为维持高能物质水平而导致的糖酵解强度的代偿性增加表现为葡萄糖摄取增加、灌注液中乳酸含量升高以及肝脏中丙酮酸激酶活性增加。NAD对的氧化还原状态（根据氧化还原代谢物含量和乳酸脱氢酶系统的平衡常数计算得出的[NAD⁺]：[NADH]比值）向肝细胞细胞质还原特性增加的方向转变。根据[ATP]：[ADP] - [Pi]比值计算得出的磷酸势值在实验条件下降低。
