Staunton M J, Gaffney E F
Department of Histopathology, St. James's Hospital, Dublin, Ireland.
Am J Clin Pathol. 1995 Mar;103(3):300-7. doi: 10.1093/ajcp/103.3.300.
Little quantitative data exist on the extent of apoptosis (genetically-mediated cell deletion) in different human tumor types. Hematoxylin and eosin-stained paraffin sections of 102 malignant tumors (58 types) were evaluated for apoptotic cells and apoptotic bodies, using the 40x objective with a calibrated eye-piece and avoiding necrotic zones. The percentage of apoptotic cells and apoptotic bodies in the total number of tumor cells examined was designated as the apoptotic index (AI) for each case. There was a wide range in the AI for different tumor types: 45 tumors had AI < 1% and 93 had an AI of < 7%. In 107 additional tumors (11 types), the AI was determined to be within the same low, intermediate, or high range as the index cases. Apoptotic nuclear material was usually more prominent than mitoses. These results suggest that each tumor type has a characteristic AI that reflects innate tumor cell susceptibility to undergo apoptosis. Additional data are needed to determine whether significant variations in AI correlate with altered proliferative indices, aberrant oncogene/tumor suppressor gene expression, and standard clinicopathologic variables.
关于不同人类肿瘤类型中细胞凋亡（基因介导的细胞清除）程度的定量数据很少。使用校准目镜的40倍物镜，避开坏死区域，对102个恶性肿瘤（58种类型）的苏木精和伊红染色石蜡切片进行凋亡细胞和凋亡小体评估。将每个病例中凋亡细胞和凋亡小体占所检查肿瘤细胞总数的百分比指定为凋亡指数（AI）。不同肿瘤类型的AI范围很广：45个肿瘤的AI<1%，93个肿瘤的AI<7%。在另外107个肿瘤（11种类型）中，AI被确定与指数病例处于相同的低、中或高范围内。凋亡核物质通常比有丝分裂更明显。这些结果表明，每种肿瘤类型都有一个特征性的AI，反映了肿瘤细胞内在的凋亡易感性。需要更多数据来确定AI的显著差异是否与增殖指数改变、癌基因/肿瘤抑制基因表达异常以及标准临床病理变量相关。
