Pickett D A, Welch D F
University Hospital, University of Oklahoma Health Sciences Center, Oklahoma City 73126.
Am J Clin Pathol. 1995 Mar;103(3):320-3. doi: 10.1093/ajcp/103.3.320.
The Organon Teknika BacT/Alert (Organon Teknika, Durham, NC), using the Pedi-BacT 20 mL aerobic bottle (BPBCS) was compared to the Wampole Isolator (WI) 1.5 Microbial tube (Wampole Laboratories, Cranbury, NJ), for detection and recovery of pediatric pathogens. The BPBCS continuously monitors culture bottles for changes in CO2 concentrations, while WI cultures are examined twice daily for appearance of colonial growth on agar media. Of 5,175 paired blood cultures, 383 pathogens were recovered from 606 positive cultures. There were 272 pathogens recovered by both systems, 64 from BPBCS only, and 47 from WI only. Overall recovery rates were 88% for BPBCS and 83% for WI. There was no significant difference between the two systems in detection or times to positivity of staphylococci, Enterobacteriaceae, or pseudomonads. Trends toward better recovery of streptococci (20 vs. 10) and fastidious microaerophiles (3 vs. 0) were found with BPBCS, whereas more slowly growing pathogens (Rochalimaea henselae [1], Mycobacterium avium-intracellulare [1]) were recovered by WI only, but because of their lower frequency did not achieve statistical significance. Detection of Haemophilus influenzae (14.9 hours in WI vs. 45.4 hours in BPBCS) was faster with WI. False positive plus contaminant cultures were detected in 5.9% BPBCS versus 1.5% WI. BPBCS offers detection of bacteremia at a rate comparable to WI with advantages of automation.
将Organon Teknika公司的BacT/Alert（位于北卡罗来纳州达勒姆的Organon Teknika公司）与Wampole Isolator（WI）1.5微生物管（位于新泽西州克兰伯里的Wampole实验室）进行比较，以检测和回收儿科病原体，其中BacT/Alert使用20 mL的儿科需氧瓶（BPBCS）。BPBCS持续监测培养瓶中二氧化碳浓度的变化，而WI培养物则每天检查两次，以观察琼脂培养基上菌落生长的情况。在5175对血培养中，从606份阳性培养物中回收了383种病原体。两个系统共回收了272种病原体，仅BPBCS回收了64种，仅WI回收了47种。BPBCS的总体回收率为88%，WI为83%。在检测葡萄球菌、肠杆菌科或假单胞菌方面，两个系统在检测或阳性时间上没有显著差异。发现BPBCS在回收链球菌（20种对10种）和苛求微需氧菌（3种对0种）方面有更好回收的趋势，而生长较慢的病原体（汉赛巴尔通体[1种]、鸟分枝杆菌复合群[1种]）仅由WI回收，但由于其出现频率较低，未达到统计学意义。WI检测流感嗜血杆菌的速度更快（WI为14.9小时，BPBCS为45.4小时）。BPBCS检测到假阳性加污染培养物的比例为5.9%，而WI为1.5%。BPBCS检测菌血症的速率与WI相当，且具有自动化的优势。
