禽成髓细胞瘤病毒DNA聚合酶对错配起始末端的利用

Utilization of mismatched initiator termini by avian myeloblastosis virus DNA polymerase.

作者信息

Weiss G B, Carr B K

出版信息

Biochim Biophys Acta. 1979 Dec 17;565(2):225-30. doi: 10.1016/0005-2787(79)90200-4.

DOI:10.1016/0005-2787(79)90200-4

PMID:92999

Abstract

DNA synthesis by avian myeloblastosis virus was studied using poly(C) as template and modified oligo(dG) as primer. The addition of one noncomplementary base to the 3'-end of the primer has no important effect on synthesis. The mispaired base is incorporated into the product and the apparent Km (for primer) and the V of the reaction remain unchanged. This confirms the absence of a 3' leads to 5'-exodeoxynuclease activity using a template that is transcribed faithfully rather than one that can undergo a slippage reaction.

摘要

利用聚（C）作为模板和修饰的寡聚（dG）作为引物，对禽成髓细胞瘤病毒的DNA合成进行了研究。在引物的3'端添加一个非互补碱基对合成没有重要影响。错配碱基被掺入产物中，反应的表观Km（对于引物）和V保持不变。这证实了在使用忠实转录而非可发生滑动反应的模板时，不存在3'到5'的外切脱氧核糖核酸酶活性。

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