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粘质沙雷氏菌的位点特异性核酸内切酶对λ噬菌体DNA的切割

Cleavage of lambda DNA by a site-specific endonuclease from Serratia marcescens.

作者信息

McParland R H, Brown L R, Pearson G D

出版信息

J Virol. 1976 Sep;19(3):1006-11. doi: 10.1128/JVI.19.3.1006-1011.1976.

DOI:10.1128/JVI.19.3.1006-1011.1976
PMID:787556
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC354941/
Abstract

Three sites recognized by SmaI endonuclease, purified from Serratia marcescens SB, have been located on lambda DNA at 0.406, 0.656, and 0.825 fractional lengths from the left end of the DNA molecule.

摘要

从粘质沙雷氏菌SB中纯化得到的SmaI核酸内切酶识别的三个位点,已定位在λ噬菌体DNA上,分别位于距DNA分子左端0.406、0.656和0.825的片段长度处。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/674b/354941/1ce1f3aca982/jvirol00225-0256-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/674b/354941/8b4961c3750b/jvirol00225-0255-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/674b/354941/1ce1f3aca982/jvirol00225-0256-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/674b/354941/8b4961c3750b/jvirol00225-0255-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/674b/354941/1ce1f3aca982/jvirol00225-0256-a.jpg

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本文引用的文献

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The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis.用聚丙烯酰胺凝胶电泳法分离高分子量核糖核酸
Biochem J. 1967 Jan;102(1):251-7. doi: 10.1042/bj1020251.
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Lambda dv: an autonomously replicating DNA fragment.λdv:一个自主复制的DNA片段。
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Characterization of polypeptides made in vitro from bacteriophage lambda DNA.λ噬菌体DNA体外合成多肽的特性分析
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Distance from cohesive end site cos determines the replication requirement for recombination in phage lambda.与粘性末端位点cos的距离决定了λ噬菌体中重组所需的复制条件。
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Mapping of restriction sites in the attachment site region of bacteriophage lambda.λ噬菌体附着位点区域限制性酶切位点的图谱绘制
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A map of the cleavage sites for endonuclease AvaI in the chromosome of bacteriophage lambda.噬菌体λ染色体中核酸内切酶AvaI切割位点的图谱。
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Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis.通过转化导入枯草芽孢杆菌的金黄色葡萄球菌质粒的特性分析。
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Transconjugant analysis: limitations on the use of sequence-specific endonucleases for plasmid identification.转接合子分析:用于质粒鉴定的序列特异性核酸内切酶使用的局限性
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Manipulation of restriction targets in phage lambda to form receptor chromosomes for DNA fragments.对噬菌体λ中的限制靶点进行操作,以形成用于DNA片段的受体染色体。
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Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI.用核酸内切酶R-RI将2型腺病毒DNA切割成六个独特的片段。
Proc Natl Acad Sci U S A. 1973 Jan;70(1):200-4. doi: 10.1073/pnas.70.1.200.
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Bacteriophage lambda having EcoRI endonuclease sites only in the nonessential region of the genome.噬菌体λ仅在基因组的非必需区域具有EcoRI核酸内切酶位点。
Proc Natl Acad Sci U S A. 1974 Oct;71(10):3927-30. doi: 10.1073/pnas.71.10.3927.
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Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis.通过琼脂糖凝胶电泳分析来自λ样噬菌体和其他病毒的DNA的核酸内切酶R-EcoRI片段。
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Nucleotide sequences at the cleavage sites of two restriction endonucleases from Hemophilus parainfluenzae.副流感嗜血杆菌两种限制性内切酶切割位点的核苷酸序列。
Biochem Biophys Res Commun. 1974 Jul 10;59(1):108-16. doi: 10.1016/s0006-291x(74)80181-6.
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DNA nucleotide sequence restricted by the RI endonuclease.受RI核酸内切酶限制的DNA核苷酸序列。
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Cleavage of DNA by R 1 restriction endonuclease generates cohesive ends.R1限制性内切核酸酶切割DNA会产生黏性末端。
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