用于检测人冠状病毒229E和OC43的巢式聚合酶链反应方法的评估
Evaluation of nested polymerase chain methods for the detection of human coronaviruses 229E and OC43.
作者信息
Myint S, Johnston S, Sanderson G, Simpson H
机构信息
Department of Child Health, University of Leicester, UK.
出版信息
Mol Cell Probes. 1994 Oct;8(5):357-64. doi: 10.1006/mcpr.1994.1052.
Abstract
Currently, the diagnosis of human respiratory coronavirus infection is either slow or insensitive. This paper describes nested polymerase chain reaction assays for the detection of human coronaviruses OC43 and 229E. The specificity and sensitivity of the assays have been determined and they have been applied to the detection of the viruses in nasal aspirates. These assays are more rapid and sensitive than cell culture and may replace the latter as the diagnostic method of choice.
摘要
目前，人类呼吸道冠状病毒感染的诊断要么耗时较长，要么不够灵敏。本文描述了用于检测人类冠状病毒OC43和229E的巢式聚合酶链反应检测方法。已确定了这些检测方法的特异性和灵敏度，并将其应用于鼻吸出物中病毒的检测。这些检测方法比细胞培养更快速、灵敏，可能会取代细胞培养成为首选的诊断方法。
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