Kelley W S, Grindley N D
Mol Gen Genet. 1976 Sep 23;147(3):307-14. doi: 10.1007/BF00582882.
Three mutations of the polA cistron, the structural gene for DNA polymerase I of E. coli, have been ordered by three factor transductional crosses. The three mutant polymerase species have altered properties which may be ascribed to defects located in different portions of the polypeptide chain. Our data indicate that the amino terminal end is encoded by the end of the polA cistron nearer to metE and that transcription and translation proceed clockwise on the E. coli circular map towards the rha locus.
通过三因子转导杂交已确定了大肠杆菌DNA聚合酶I的结构基因polA顺反子的三个突变。这三种突变型聚合酶具有改变的特性，这些特性可能归因于位于多肽链不同部分的缺陷。我们的数据表明，氨基末端由polA顺反子中更靠近metE的一端编码，并且转录和翻译在大肠杆菌环形图谱上朝着rha位点顺时针进行。
