Khalil A, Ayoub M, el-Din Abdel-Wahab K S, el-Salakawy A
Research Institute of Ophthalmology, Cairo, Egypt.
Br J Ophthalmol. 1995 Jan;79(1):6-9. doi: 10.1136/bjo.79.1.6.
Sixty one corneas taken from 33 hepatitis B surface antigen (HBsAg) seropositive donors and 20 control corneas taken from 12 HBsAg seronegative donors were tested for the presence of HBsAg using reversed passive haemagglutination (RPHA) and enzyme linked immunosorbent assay (ELISA) and for the presence of hepatitis B virus core DNA (HBVcDNA) using the hybridisation technique in their epithelium, stroma endothelium, and storage media. HBsAg was detected by ELISA in the epithelium of one cornea (1.6%), in the stroma endothelial suspensions of six corneas (9.8%), and in the storage media of five corneas (8.2%). HBVcDNA was detected for the first time in the cornea; in the epithelium of four corneas (6.6%), stroma endothelium of nine corneas (14.8%), and the storage media of five corneas (8.2%). The control corneas were negative for HBsAg, while HBVcDNA was detected in the stroma endothelium of two corneas (10%) and in the media of two corneas (10%). This study confirmed that HBV can be present in the human cornea. Preservation in corneal storage media for up to 6 days could not eliminate the virus from the cornea. The possibility of HBV transmission through corneal transplantation should not be overlooked.
对取自33名乙肝表面抗原（HBsAg）血清阳性供体的61只角膜以及取自12名HBsAg血清阴性供体的20只对照角膜，采用反向被动血凝试验（RPHA）和酶联免疫吸附测定（ELISA）检测HBsAg的存在情况，并采用杂交技术检测其上皮、基质、内皮及保存介质中乙肝病毒核心DNA（HBVcDNA）的存在情况。通过ELISA检测发现，一只角膜的上皮（1.6%）、6只角膜的基质内皮悬液（9.8%）以及5只角膜的保存介质（8.2%）中存在HBsAg。首次在角膜中检测到HBVcDNA；4只角膜的上皮（6.6%）、9只角膜的基质内皮（14.8%）以及5只角膜的保存介质（8.2%）中存在HBVcDNA。对照角膜的HBsAg检测呈阴性，而在2只角膜的基质内皮（10%）和2只角膜的介质（10%）中检测到HBVcDNA。本研究证实HBV可存在于人类角膜中。角膜在保存介质中保存长达6天并不能消除角膜中的病毒。不应忽视HBV通过角膜移植传播的可能性。
