Nasr F, Bécam A M, Slonimski P P, Herbert C J
Centre de Génétique Moléculaire, Laboratoire propre du CNRS, l'Université Pierre-et-Marie-Curie, Gif-sur-Yvette, France.
C R Acad Sci III. 1994 Jul;317(7):607-13.
The gene YBR1012 was identified during the systematic sequencing of chromosome II of the yeast Saccharomyces cerevisiae. We have inactivated the gene and shown that it is essential for cellular viability. Using antisense RNA technology we have constructed a conditional allele, expression of the antisense RNA strongly inhibits growth. To our knowledge this is the first successful use of antisense RNA technology in S. cerevisiae. Comparison of the deduced ybr1012p sequence with the data banks revealed the presence of a putative phosphatidylinositol kinase domain and a strong homology to the Schizosaccharomyces pombe rad3p. These results suggest that ybr1012p may be involved in signal transduction, possibly related to the control of replication and/or DNA damage repair. The link with DNA damage repair was reinforced by the isolation of the DUN1 gene as a multicopy suppressor of the YBR1012 deletion.
基因YBR1012是在对酿酒酵母二号染色体进行系统测序时被鉴定出来的。我们已使该基因失活，并证明它对细胞活力至关重要。利用反义RNA技术，我们构建了一个条件等位基因，反义RNA的表达强烈抑制生长。据我们所知，这是反义RNA技术在酿酒酵母中的首次成功应用。将推导的ybr1012p序列与数据库进行比较，发现存在一个假定的磷脂酰肌醇激酶结构域，并且与粟酒裂殖酵母rad3p有很强的同源性。这些结果表明，ybr1012p可能参与信号转导，可能与复制控制和/或DNA损伤修复有关。通过分离DUN1基因作为YBR1012缺失的多拷贝抑制子，加强了与DNA损伤修复的联系。
